Mammalian steroid hormones are substrates for the major RND- and MFS-type tripartite multidrug efflux pumps of Escherichia coli

被引:49
作者
Elkins, CA [1 ]
Mullis, LB [1 ]
机构
[1] US FDA, Div Microbiol, Natl Ctr Toxicol Res, Jefferson, AR 72079 USA
关键词
D O I
10.1128/JB.188.3.1191-1195.2006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A steroid-hormone-dependent growth suppression was observed in Escherichia coli efflux-deficient backgrounds containing mutations in the major RND- and MFS-type tripartite multidrug efflux systems, AcrAB-TolC and EmrAB-TolC, respectively. In addition to their previously known natural steroid spectrum, which includes bile acids, both systems were shown to transport the hormones estradiol and progesterone, whereas hydrocortisone served as a substrate of only AcrAB-TolC. Furthermore, at least two other RND-type pumps, YhiV and AcrD, were capable of transporting such hormones when overexpressed on plasmid vectors (with some demonstrable specificity observed with AcrD). When this activity was examined in a wild-type background, cell-associated estradiol levels remained largely unaffected by competition with exogenous bile acids and hydrocortisone, in contrast to progesterone, which produced a significant modulation in estradiol uptake.
引用
收藏
页码:1191 / 1195
页数:5
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