Enzymatic conversion of cephalosporin C into glutaryl 7-aminocephalosporanic acid. A study in different reactor configurations

被引：18

作者：

Alfani, F ^{[1
]}

Cantarella, M ^{[1
]}

Cutarella, N ^{[1
]}

Gallifuoco, A ^{[1
]}

Golini, P ^{[1
]}

Bianchi, D ^{[1
]}

机构：

[1] ENICHEM SPA,IST G DONEGANI,I-28100 NOVARA,ITALY

来源：

BIOTECHNOLOGY LETTERS | 1997年 / 19卷 / 02期

关键词：

D O I：

10.1023/A:1018324700707

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Conversion of cephalosporin C into glutaryl 7-aminocephalosporanic acid was catalysed by D-aminoacid oxidase from Trigonopsis variabilis, covalently immobilized on the polystyrenic resin Duolite A365. Spontaneous degradation of substrates was limited without depressing enzymatic activity at the optimum reaction pH 8.0. The highest product yield was 1.77 mmol per g of biocatalyst, attained at 15 degrees C in both batch stirred and fluidized-bed reactors.

引用

页码：175 / 178

页数：4

共 6 条

[1] PRELIMINARY INVESTIGATION FOR THE CONTINUOUS ENZYMATIC PRODUCTION OF 7-AMINOCEPHALOSPORANIC ACID [J].