Conditional regulation of a ROCK-estrogen receptor fusion protein

被引：19

作者：

Croft, DR ^{[1
]}

Olson, MF ^{[1
]}

机构：

[1] Beatson Inst Canc Res, Glasgow G61 1BD, Lanark, Scotland

来源：

METHODS IN ENZYMOLOGY, VOL 406, REGULATORS AND EFFECTORS OF SMALL GTPASES: RHO FAMILY | 2006年 / 406卷

关键词：

D O I：

10.1016/S0076-6879(06)06042-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The Rho-associated kinases ROCK I and ROCK II are serine/threonine kinases that play central and critical roles in regulating the actin cytoskeleton. Activation of ROCK proteins contributes positively to the phosphorylation of myosin II light chains (MLC), myosin ATPase activity, stabilization of filamentous actin, and coupling of the actin-myosin filaments to the plasma membrane, thereby leading to the increased actin-myosin force generation and contractility. We have constructed a conditionally-activated form of ROCK II (called ROCKER) by fusing the ROCK II kinase domain to the estrogen receptor hormone-binding domain. In this chapter, we describe the construction and characterization of this regulatable ROCKER fusion protein.

引用

页码：541 / 553

页数：13

共 19 条

[11] Keratinocyte differentiation is regulated by the Rho and ROCK signaling pathway [J].

McMullan, R ;

Lax, S ;

Robertson, VH ;

Radford, DJ ;

Broad, S ;

Watt, FM ;

Rowles, A ;

Croft, DR ;

Olson, MF ;

Hotchin, NA .

CURRENT BIOLOGY, 2003, 13 (24) :2185-2189

[12] Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop [J].

Ohashi, K ;

Nagata, K ;

Maekawa, M ;

Ishizaki, T ;

Narumiya, S ;

Mizuno, K .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (05) :3577-3582

[13] PRODUCTION OF HIGH-TITER HELPER-FREE RETROVIRUSES BY TRANSIENT TRANSFECTION [J].

PEAR, WS ;

NOLAN, GP ;

SCOTT, ML ;

BALTIMORE, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (18) :8392-8396

[14] Rocks: Multifunctional kinases in cell behaviour [J].

Riento, K ;

Ridley, AJ .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2003, 4 (06) :446-456

[15] Transient transfection of ecotropic retrovirus receptor permits stable gene transfer into non-rodent cells with murine retroviral vectors [J].

Scholz, A ;

Beato, M .

NUCLEIC ACIDS RESEARCH, 1996, 24 (05) :979-980

[16] Distinct roles of ROCK (Rho-kinase) and MLCK in spatial regulation of MLC phosphorylation for assembly of stress fibers and focal adhesions in 3T3 fibroblasts [J].

Totsukawa, G ;

Yamakita, Y ;

Yamashiro, S ;

Hartshorne, DJ ;

Sasaki, Y ;

Matsumura, F .

JOURNAL OF CELL BIOLOGY, 2000, 150 (04) :797-806

[17] Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension [J].

Uehata, M ;

Ishizaki, T ;

Satoh, H ;

Ono, T ;

Kawahara, T ;

Morishita, T ;

Tamakawa, H ;

Yamagami, K ;

Inui, J ;

Maekawa, M ;

Narumiya, S .

NATURE, 1997, 389 (6654) :990-994

[18] Rho/Rho-kinase mediated signaling in physiology and pathophysiology [J].

Wettschureck, N ;

Offermanns, S .

JOURNAL OF MOLECULAR MEDICINE-JMM, 2002, 80 (10) :629-638

[19] Raf-induced proliferation or cell cycle arrest is determined by the level of Raf activity with arrest mediated by p21(Cip1) [J].

Woods, D ;

Parry, D ;

Cherwinski, H ;

Bosch, E ;

Lees, E ;

McMahon, M .

MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (09) :5598-5611

← 1 2 →