Associations between the presence and magnitude of Escherichia coli O157 in feces at harvest and contamination of preintervention beef carcasses

被引:41
作者
Fox, J. T. [1 ]
Renter, D. G. [1 ]
Sanderson, M. W. [2 ]
Nutsch, A. L. [3 ]
Shi, X. [1 ]
Nagaraja, T. G. [1 ]
机构
[1] Kansas State Univ, Dept Diagnost Med & Pathobiol, Manhattan, KS 66506 USA
[2] Kansas State Univ, Coll Vet Med, Dept Clin Sci, Manhattan, KS 66506 USA
[3] Kansas State Univ, Inst Food Sci, Dept Anim Sci & Ind, Manhattan, KS 66506 USA
关键词
D O I
10.4315/0362-028X-71.9.1761
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
To quantify associations at slaughter between Escherichia coli O157 carcass contamination, fecal-positive animals, and high-shedding animals within truckloads of finished cattle, we sampled up to 32 cattle from each of 50 truckloads arriving at a commercial abattoir in the Midwest United States during a 5-week summer period. Carcass swab samples collected preevisceration and fecal samples collected postevisceration were matched within animals and analyzed for the presence of E. coli O157, using enrichment, immunomagnetic separation, and plating on selective media (IMS). In addition, a direct plating procedure was performed on feces to identify high-shedding animals. E. coli O157 was isolated from 39 (2.6%) of 1,503 carcass samples in 15 (30%) truckloads, and 127 (8.5%) of 1,495 fecal samples in 37 (74%) truckloads. Fifty-five (3.7%) high-shedding animals were detected from 26 (52%) truckloads. Truckload high-shedder (Spearman rank-order correlation coefficient [r(s)] = 0.68), IMS-positive (r(s) = 0.48), and combined fecal (r(s) = 0.61) prevalence were significantly correlated with carcass prevalence. The probability of isolating E. coli O157 from a carcass was not significantly associated with the high-shedder or fecal IMS status of the animal from which the carcass was derived. However, the probability of carcass contamination was significantly associated with all truckload-level measures of fecal E. coli 0 157, particularly whether or not a high shedder was present within the truckload (odds ratio = 16.2; 95% confidence interval, 6.3-43.6). Our results suggest that high shedders within a truckload at slaughter could be a target for mitigation strategies to reduce the probability of preevisceration carcass contamination.
引用
收藏
页码:1761 / 1767
页数:7
相关论文
共 28 条
[1]   Transportation and lairage environment effects on prevalence, numbers, and diversity of Escherichia coli O157:H7 on hides and carcasses of beef cattle at processing [J].
Arthur, Terrance M. ;
Bosilevac, Joseph M. ;
Brichta-Harhay, Dayna M. ;
Guerini, Michael N. ;
Kalchayanand, Norasak ;
Shackelford, Steven D. ;
Wheeler, Tommy L. ;
Koohmaraie, Mohammad .
JOURNAL OF FOOD PROTECTION, 2007, 70 (02) :280-286
[2]   Escherichia coli O157 prevalence and enumeration of aerobic bacteria, Enterobacteriaceae, and Escherichia coli O157 at various steps in commercial beef processing plants [J].
Arthur, TM ;
Bosilevac, JM ;
Nou, XW ;
Shackelford, SD ;
Wheeler, TL ;
Kent, MP ;
Jaroni, D ;
Pauling, B ;
Allen, DM ;
Koohmaraie, M .
JOURNAL OF FOOD PROTECTION, 2004, 67 (04) :658-665
[3]   Seasonal prevalence of Shiga toxin-producing Escherichia coli, including O157:H7 and non-O157 serotypes, and Salmonella in commercial beef processing plants [J].
Barkocy-Gallagher, GA ;
Arthur, TM ;
Rivera-Betancourt, M ;
Nou, XW ;
Shackelford, SD ;
Wheeler, TL ;
Koohmaraie, M .
JOURNAL OF FOOD PROTECTION, 2003, 66 (11) :1978-1986
[4]   Genotypic analyses of Escherichia coli O157:H7 and O157 nonmotile isolates recovered from beef cattle and carcasses at processing plants in the midwestern states of the United States [J].
Barkocy-Gallagher, GA ;
Arthur, TM ;
Siragusa, GR ;
Keen, JE ;
Elder, RO ;
Laegreid, WW ;
Koohmaraie, M .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (09) :3810-3818
[5]   The effects of preslaughter washing on the reduction of Escherichia coli O157:H7 transfer from cattle hides to carcasses during slaughter [J].
Byrne, CM ;
Bolton, DJ ;
Sheridan, JJ ;
McDowell, DA ;
Blair, IS .
LETTERS IN APPLIED MICROBIOLOGY, 2000, 30 (02) :142-145
[6]   CATTLE AS A POSSIBLE SOURCE OF VEROCYTOTOXIN-PRODUCING ESCHERICHIA-COLI-O157 INFECTIONS IN MAN [J].
CHAPMAN, PA ;
WRIGHT, DJ ;
NORMAN, P ;
FOX, J ;
CRICK, E .
EPIDEMIOLOGY AND INFECTION, 1993, 111 (03) :439-447
[7]   Rectoanal junction colonization of feedlot cattle by Escherichia coli O157:H7 and its association with supershedders and excretion dynamics [J].
Cobbold, Rowland N. ;
Hancock, Dale D. ;
Rice, Daniel H. ;
Berg, Janice ;
Stilborn, Robert ;
Hovde, Carolyn J. ;
Besser, Thomas E. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (05) :1563-1568
[8]  
Cosenza-Sutton GH, 2004, THESIS U FLORIDAA
[9]   Correlation of enterohemorrhagic Escherichia coli O157 prevalence in feces, hides, and carcasses of beef cattle during processing [J].
Elder, RO ;
Keen, JE ;
Siragusa, GR ;
Barkocy-Gallagher, GA ;
Koohmaraie, M ;
Laegreid, WW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (07) :2999-3003
[10]   An investigation of Escherichia coli O157 contamination of cattle during slaughter at an abattoir [J].
Fegan, N ;
Higgs, G ;
Vanderlinde, P ;
Desmarchelier, P .
JOURNAL OF FOOD PROTECTION, 2005, 68 (03) :451-457