Determination of Schistosoma japonicum circulating antigens in dilution serum by piezoelectric immunosensor and S/N enhancement

被引：13

作者：

Cheng, Chien-Wei ^{[1
]}

Chen, Ching-Kuo ^{[2
,3
]}

Chen, Yi-Shen ^{[1
]}

Chen, Liang-Yu ^{[1
]}

机构：

[1] Ming Chuan Univ, Dept Biotechnol, Gui Shan 333, Taiwan

[2] Natl Cent Univ, Dept Chem & Mat Engn, Jhongli 320, Taiwan

[3] Natl Taipei Univ Technol, Dept Chem Engn & Biotechnol, Taipei 106, Taiwan

来源：

BIOSENSORS & BIOELECTRONICS | 2008年 / 24卷 / 01期

关键词：

piezoelectric sensor; Schistosomiasis; circulating antigens; QCM;

D O I：

10.1016/j.bios.2008.03.029

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A piezoelectric immunosensor assay was developed with immobilizing immunoglobulin G (IgG) as a probe to detect Schistosoma japonicum circulating antigens (SjCAg). Analytical strategy utilizes the polyclonal antibodies with broad-spectrum recognition to a complex target with high specificity. The immobilized antibodies were purified from immunized rabbit's sera (im-S) and infected rabbit's sera (inf-S) by S.japonicum. The detection capacities of antibodies were compared between the sera of different phenotypes and purified fractions. The sample dilution ratios were also evaluated and optimized. Additionally, the sera with a variety of infection degrees for validation could be discriminated quantitatively. The linear dose-response relationship indicates that the systematic sensitivity of this method is below 150 Hz and the lowest limit of detectable range is above 500 cercariae of S.japonicum infection for 2 weeks. The novel immunosensor technique is well potential to determine the SjCAg in serum samples for clinical diagnosis of parasitosis in early stage. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：136 / 140

页数：5

共 28 条

[1] SCHISTOSOMA-MANSONI - DETECTION AND CHARACTERIZATION OF SCHISTOSOME DERIVED ANTIGENS BY INHIBITION ENZYME-LINKED IMMUNOSORBENT-ASSAY (IELISA) UTILIZING MONOCLONAL-ANTIBODIES [J].