Multiple splicing variants of cdc25B regulate G2/M progression

被引：58

作者：

Forrest, ARR

McCormack, AK

DeSouza, CPC

Sinnamon, JM

Tonks, ID

Hayward, NK

Ellem, KAO

Gabrielli, BG ^{[1
]}

机构：

[1] Queensland Inst Med Res, Queensland Canc Fund Res Labs, Brisbane, Qld 4029, Australia

[2] Univ Queensland, Joint Oncol Program, Brisbane, Qld 4029, Australia

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 260卷 / 02期

基金：

英国医学研究理事会; 澳大利亚研究理事会;

关键词：

D O I：

10.1006/bbrc.1999.0870

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Progression through G2 phase into mitosis is regulated by the activation of the mitotic cyclin/cdk complexes, which are in turn activated cdc25B and cdc25C phosphatases. Here we report that alternate splicing produces at least five variants of cdc25B, although only cdc25B2 and cdc25B3 are detectable as proteins. Analysis of these two variants shows that cdc25B2 is expressed at lower levels relative to cdc25B3 in all cell lines tested, and the expression of both increased markedly during G2 and mitosis. Overexpression of the catalytically inactive version of either cdc25B variant produced a G2 arrest implicating both in regulating G2/M progression. (C) 1999 Academic Press.

引用

页码：510 / 515

页数：6

共 29 条

[1] Alternative splicing of the human CDC25B tyrosine phosphatase. Possible implications for growth control? [J].