DEVELOPMENT OF MICROSATELLITE MARKERS IN ILEX KAUSHUE (AQUIFOLIACEAE), A MEDICINAL PLANT SPECIES

被引：1

作者：

Qin, Lan-Fang ^{[1
]}

Qu, Xin-Cheng ^{[1
]}

Hu, Gang ^{[2
]}

Huang, Yun-Feng ^{[1
]}

Zhang, Qi-Wei ^{[1
]}

机构：

[1] Guangxi Inst Tradit Med & Pharmaceut Sci, Naning 530022, Peoples R China

[2] Guangxi Teachers Educ Univ, Sch Environm & Life Sci, Naning 530001, Peoples R China

来源：

APPLICATIONS IN PLANT SCIENCES | 2015年 / 3卷 / 08期

关键词：

Aquifoliaceae; genetic diversity; Ilex kaushue; microsatellite marker;

D O I：

10.3732/apps.1500040

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Premise of the study: Microsatellite markers were developed for Ilex kaushue (Aquifoliaceae), a medicinal plant with extremely small wild populations that exists in fragmented habitats, to assess and protect its genetic diversity. Methods and Results: Using 454 GS FLX Titanium sequencing, 16 microsatellite primer sets were isolated and characterized. Fifteen of these markers were polymorphic. The number of alleles per locus ranged from one to nine across 22 individuals from both cultivated and wild populations. The observed and expected heterozygosity in these two populations ranged from 0.000 to 1.000 and from 0.000 to 0.785, respectively. Conclusions: These markers will be useful in studies on genetic diversity of I. kaushue

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