Environmental influences on Actinobacillus actinomycetemcomitans biofilm formation

被引:26
作者
Haase, EM
Bonstein, T
Palmer, RJ
Scannapieco, FA
机构
[1] SUNY Buffalo, Dept Oral Biol, Buffalo, NY 14214 USA
[2] SUNY Buffalo, Dept Restorat Dent, Buffalo, NY 14214 USA
[3] Univ Tennessee, Ctr Environm Biotechnol, Knoxville, TN 37932 USA
关键词
biofilm; Actinobacillus actinomycetemcomitans; flowcell; microtitre plate assay;
D O I
10.1016/j.archoralbio.2005.09.002
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Fresh clinical isolates of the periodontal. pathogen Actinobacillus actinomycetemcomitans have an adherent, rough colony morphology that transforms into a minimally adherent, smooth colony phenotype during successive in vitro passage. The objectives of this study were: (1) to compare biofilm formation of the rough (RVs) and smooth variants (SVs) of several strains of A. actinomycetemcomitons grown under various environmental conditions and (2) to examine the dynamics of biofilm formation. A microtitre plate biofilm assay was used to evaluate biofilm formation of strains grown in broth with modified salt concentration and pH, and to evaluate the effect of pre-conditioning films. Scanning electron microscopy (SEM) was used to monitor microscopic changes in morphology. Dynamics of biofilm formation were measured in a flowcell monitored by confocal microscopy. The RVs generally produced greater biofilm than the SVs. However, medium-dependent differences in biofilm formation were evident for some rough/smooth pairs. The RVs were more tolerant to changes in salt and pH, and more resistant to chlorhexidine than the SVs. Horse serum virtually eliminated, and saliva significantly reduced, biofilm formation by the SVs in contrast to the RVs. SEM revealed no alteration in morphology with change of environment. In a flowcell, the RVs produced towers of microcolonies anchored by a small contact area, whereas the SVs produced an open architecture of reduced height. After 7 days in a flowcell, the rough to smooth phenotype transition could be demonstrated. In conclusion, strain, growth medium and conditioning film all affect biofilm formation. The RVs produce biofilms of unique architecture that may serve to protect the bacterium from environmental perturbations. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:299 / 314
页数:16
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