Isolation and characterization of novel microsatellite markers from the Australian water skink Eulamprus kosciuskoi and cross-species amplification in other members of the species-group

被引：21

作者：

Scott, IAW ^{[1
]}

Hayes, CM ^{[1
]}

Keogh, JS ^{[1
]}

Morrison, SF ^{[1
]}

机构：

[1] Australian Natl Univ, Div Bot & Zool, Canberra, ACT 0200, Australia

来源：

MOLECULAR ECOLOGY NOTES | 2001年 / 1卷 / 1-2期

关键词：

Eulamprus; lizard; mating system; microsatellite; Sphenomorphus group;

D O I：

10.1046/j.1471-8278.2000.00011.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A panel of six samples from three Eulampnis species was used to screen 24 primer pairs developed from the Australian water skink Eulampnis koschiskoi. We provide details of 10 microsatellite loci that exhibited the cleanest and strongest banding patterns. We also screened six of these microsatellite loci against 118 individuals from a population of E. heatwolei. These microsatellites exhibited large numbers of alleles per locus (4-19) and high heterozygosity (0.271-0.898) in this population. This suggests that they will be extremely useful for investigating mating systems and other facets of population biology in these lizards.

引用

页码：28 / 30

页数：3