Biosynthesis of poly(3-hydroxybutyrate-co-3-mercaptobutyrate) as a sulfur analogue to poly(3-hydroxybutyrate) (PHB)

被引:67
作者
Lütke-Eversloh, T
Bergander, K
Luftmann, H
Steinbüchel, A
机构
[1] Univ Munster, Inst Mikrobiol, D-48149 Munster, Germany
[2] Univ Munster, Inst Organ Chem, D-48149 Munster, Germany
关键词
D O I
10.1021/bm015564p
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A hitherto unknown copolymer that contains sulfur in the backbone linking 3-hydroxybutyrate and 3-mercaptobutyrate by thioester linkages, poly(3HB-co-3MB), was synthesized by the polyhydroxyalkanoate(PHA-) accumulating bacterium Ralstonia eutropha, when 3-mercaptobutyric acid was fed as the carbon source in addition to gluconate. The chemical structure of this novel polymer was confirmed by gas chromatography/mass spectrometry, infrared spectroscopy, H-1 and C-13 nuclear magnetic resonance spectroscopy, and elemental sulfur analysis. Different poly(3HB-co-3MB) samples were isolated and characterized, and the molar 3MB fraction contributed up to 72 mol %. Regarding the chemical structure of 3-mercaptobutyrate, which is analogous to 3-hydroxybutyrate, the incorporation of this novel polymer constituent represents a second example of 3-mercaptoalkanoates that are incorporated into biopolymers by the PHA synthase of R. eutropha.
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页码:1061 / 1065
页数:5
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