Heteronuclear NMR studies of human serum apolipoprotein A-I. Part I. Secondary structure in lipid-mimetic solution

被引:32
作者
Okon, M
Frank, PG
Marcel, YL
Cushley, RJ
机构
[1] Simon Fraser Univ, Dept Mol Biol & Biochem, Burnaby, BC V5A 1S6, Canada
[2] Univ Ottawa, Inst Heart, Lipoprot & Atherosclerosis Res Grp, Ottawa, ON K1Y 4E9, Canada
基金
英国医学研究理事会; 加拿大自然科学与工程研究理事会;
关键词
protein structure; apolipoprotein; nuclear magnetic resonance; chemical shift index; torsion angle likelihood obtained from shift and sequence similarity; sodium dodecyl sulfate;
D O I
10.1016/S0014-5793(02)02600-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The apolipoprotein A-I (apoA-I) solution structure in the presence of sodium dodecyl sulfate (SDS) was determined by combination of chemical shift index and torsion angle likelihood obtained from shift and sequence similarity methods. ApoA-I in lipid-mimetic solution is composed of alpha-helices (residues 8-32, 45-64, 67-77, 82-86, 90-97, 100-118, 122-140, 146-162, 167205, 210-216 and 221-239), with 2-5 residue irregular segments between helical repeats, and the irregular segment 78-81 within helical repeat 2. ApoA-I is a monomer in the SDS complex and no evidence of interhelical interactions is found. Comparison of the apoA-I and apoA-I(1-186) [Okon et al., FEBS Lett. 487 (2001) 390-396] solution structures revealed that apoA-I undergoes a conformational change around Pro121. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
引用
收藏
页码:139 / 143
页数:5
相关论文
共 39 条
[1]   Identification of scavenger receptor SR-BI as a high density lipoprotein receptor [J].
Acton, S ;
Rigotti, A ;
Landschulz, KT ;
Xu, SZ ;
Hobbs, HH ;
Krieger, M .
SCIENCE, 1996, 271 (5248) :518-520
[2]   OPTIMIZED RECORDING OF HETERONUCLEAR MULTIDIMENSIONAL NMR-SPECTRA USING PULSED FIELD GRADIENTS [J].
BAX, A ;
POCHAPSKY, SS .
JOURNAL OF MAGNETIC RESONANCE, 1992, 99 (03) :638-643
[3]   Characterization of human apolipoprotein A-I expressed in Escherichia coli [J].
Bergeron, J ;
Frank, PG ;
Emmanuel, F ;
Latta, M ;
Zhao, YW ;
Sparks, DL ;
Rassart, E ;
Denefle, P ;
Marcel, YL .
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, 1997, 1344 (02) :139-152
[4]   Crystal structure of truncated human apolipoprotein A-I suggests a lipid-bound conformation [J].
Borhani, DW ;
Rogers, DP ;
Engler, JA ;
Brouillette, CG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (23) :12291-12296
[5]   Deletion of the C-terminal domain of apolipoprotein A-I impairs cell surface binding and lipid efflux in macrophage [J].
Burgess, JW ;
Frank, PG ;
Franklin, V ;
Liang, P ;
McManus, DC ;
Desforges, M ;
Rassart, E ;
Marcel, YL .
BIOCHEMISTRY, 1999, 38 (44) :14524-14533
[6]   APOLIPOPROTEIN-A-I CONFORMATION IN DISCOIDAL PARTICLES - EVIDENCE FOR ALTERNATE STRUCTURES [J].
CALABRESI, L ;
MENG, QH ;
CASTRO, GR ;
MARCEL, YL .
BIOCHEMISTRY, 1993, 32 (25) :6477-6484
[7]   EARLY INCORPORATION OF CELL-DERIVED CHOLESTEROL INTO PRE-BETA-MIGRATING HIGH-DENSITY LIPOPROTEIN [J].
CASTRO, GR ;
FIELDING, CJ .
BIOCHEMISTRY, 1988, 27 (01) :25-29
[8]   SUPPRESSION OF CROSS-RELAXATION EFFECTS IN TOCSY SPECTRA VIA A MODIFIED DIPSI-2 MIXING SEQUENCE [J].
CAVANAGH, J ;
RANCE, M .
JOURNAL OF MAGNETIC RESONANCE, 1992, 96 (03) :670-678
[9]   4-DIMENSIONAL C-13/C-13-EDITED NUCLEAR OVERHAUSER ENHANCEMENT SPECTROSCOPY OF A PROTEIN IN SOLUTION - APPLICATION TO INTERLEUKIN 1-BETA [J].
CLORE, GM ;
KAY, LE ;
BAX, A ;
GRONENBORN, AM .
BIOCHEMISTRY, 1991, 30 (01) :12-18
[10]   Protein backbone angle restraints from searching a database for chemical shift and sequence homology [J].
Cornilescu, G ;
Delaglio, F ;
Bax, A .
JOURNAL OF BIOMOLECULAR NMR, 1999, 13 (03) :289-302