Development of the one-on-one quality assessment assay for entomopathogenic nematodes

被引：21

作者：

Converse, V ^{[1
]}

Miller, RW ^{[1
]}

机构：

[1] Univ Adelaide, Dept Appl & Mol Biol, Glen Osmond, SA 5064, Australia

来源：

JOURNAL OF INVERTEBRATE PATHOLOGY | 1999年 / 74卷 / 02期

关键词：

bioassay; entomopathogenic nematodes; Galleria mellonella; one-on-one assay; steinernematid; Steinernema carpocapsae; S; feltiae; glaseri; riobrave; virulence profiles; quality assessment;

D O I：

10.1006/jipa.1999.4867

中图分类号：

Q95 [动物学];

学科分类号：

071002 [动物学];

摘要：

The one-on-one bioassay was developed using Steinernema carpocapsae (All) nematodes against the wax moth larva, Galleria mellonella. The assay was used to develop and compare virulence profiles of both in vitro- and in vivo-produced nematodes and to provide a quality assessment 'standard' for in vitro-produced nematodes. The bioassay was subsequently used to develop virulence profiles for Steinernema carpocapsae (UK), S. feltiae (UK), S. feltiae (R1.5), S. feltiae (SN), S. glaseri (NJ-43), and S. riobrave (RGV). These profiles are unique for each species and isolate and are used as a standard of virulence in routine quality assessment of nematodes produced in liquid fermentation. (C) 1999 Academic Press.

引用

页码：143 / 148

页数：6

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