Separation and purification of isoflavones from Pueraria lobata by high-speed counter-current chromatography

被引：115

作者：

Cao, XL

Tian, Y

Zhang, TY

Li, X

Ito, Y

机构：

[1] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA

[2] Beijing Inst New Technol Applicat, Beijing 100035, Peoples R China

[3] Beijing Secondary Pharmaceut Factory Tradit Chine, Beijing 100010, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 855卷 / 02期

关键词：

Pueraria lobata; pharmaceutical analysis; plant materials; isoflavones; puerarins;

D O I：

10.1016/S0021-9673(99)00715-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-speed counter-current chromatography (HSCCC) was applied to the semipreparative separation and purification of puerarin and related isoflavones from a crude extract of Pueraria lobata. Analytical HSCCC was used for the preliminary selection of a suitable solvent system composed of ethyl acetate-n-butanol-water (2:1.3, v/v/v). Using the above solvent system the preparative HSCCC was successfully performed yielding six relatively pure isoflavones including puerarin from 80 mg of the crude extract in one-step separation. (C) 1999 Elsevier Science BN. All rights reserved.

引用

页码：709 / 713

页数：5