A murine dopamine neuron-specific cDNA library and microarray: Increased COXI expression during methamphetamine neurotoxicity

被引：26

作者：

Barrett, T

Xie, T

Piao, Y

Dillon-Carter, O

Kargul, GJ

Lim, MK

Chrest, FJ

Wersto, R

Rowley, DL

Juhaszova, M

Zhou, L

Vawter, MP

Becker, KG

Cheadle, C

Wood, WH

McCann, UD

Freed, WJ

Ko, MS

Ricaurte, GA

Donovan, DM

机构：

[1] NIA, Intramural Res Program, Res Resources Branch, NIH, Baltimore, MD 21224 USA

[2] NIA, Genet Lab, NIH, Baltimore, MD 21224 USA

[3] NIDA, Cellular Neurobiol Branch, NIH, Baltimore, MD 21224 USA

[4] Johns Hopkins Univ, Sch Med, Dept Neurol, Baltimore, MD 21205 USA

[5] Johns Hopkins Univ, Sch Med, Dept Psychiat & Behav Sci, Baltimore, MD 21205 USA

来源：

NEUROBIOLOGY OF DISEASE | 2001年 / 8卷 / 05期

关键词：

dopamine; cDNA library; cDNA microarray; methamphetamine; development; gene expression profile;

D O I：

10.1006/nbdi.2001.0423

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Due to brain tissue heterogeneity, the molecular genetic profile of any neurotransmitter-specific neuronal subtype is unknown. The purpose of this study was to purify a population of dopamine neurons, construct a cDNA library, and generate an initial gene expression profile and a microarray representative of dopamine neuron transcripts. Ventral mesencephalic dopamine neurons were purified by fluorescent-activated cell sorting from embryonic day 13.5 transgenic mice harboring a 4.5-kb rat tyrosine hydroxylase promoter-lacZ fusion. Nine-hundred sixty dopamine neuron cDNA clones were sequenced and arrayed for use in studies of gene expression changes during methamphetamine neurotoxicity. A neurotoxic dose of methamphetamine produced a greater than twofold up-regulation of the mitochondrial cytochrome c oxidase polypeptide I transcript from adult mouse substantial nigra at 12 h posttreatment. This is the first work to describe a gene expression profile for a neuronal subtype and to identify gene expression changes during methamphetamine neurotoxicity. (C) 2001 Academic Press.

引用

页码：822 / 833

页数：12

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