In vivo near-infrared fluorescence imaging of osteoblastic activity

被引：306

作者：

Zaheer, A

Lenkinski, RE

Mahmood, A

Jones, AG

Cantley, LC

Frangioni, JV ^{[1
]}

机构：

[1] Beth Israel Deaconess Med Ctr, Dept Med, Div Hematol Oncol, Boston, MA 02215 USA

[2] Beth Israel Deaconess Med Ctr, Dept Radiol, Boston, MA 02215 USA

[3] Brigham & Womens Hosp, Dept Radiol, Boston, MA 02115 USA

[4] Harvard Univ, Sch Med, Boston, MA 02215 USA

[5] Beth Israel Deaconess Med Ctr, Dept Med, Div Signal Transduct, Boston, MA 02215 USA

来源：

NATURE BIOTECHNOLOGY | 2001年 / 19卷 / 12期

关键词：

D O I：

10.1038/nbt1201-1148

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In vertebrates, the development and integrity of the skeleton requires hydroxyapatite (HA) deposition by osteoblasts. HA deposition is also a marker of, or a participant in, processes as diverse as cancer and atherosclerosis. At present, sites of osteoblastic activity can only be imaged in vivo using gamma -emitting radioisotopes. The scan times required are long, and the resultant radioscintigraphic images suffer from relatively low resolution. We have synthesized a near-infrared (NIR) fluorescent bisphosphonate derivative that exhibits rapid and specific binding to HA in vitro and in vivo. We demonstrate NIR light-based detection of osteoblastic activity in the living animal, and discuss how this technology can be used to study skeletal development, osteoblastic metastasis, coronary atherosclerosis, and other human diseases.

引用

页码：1148 / 1154

页数：7

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[1] Indocyanine green (ICG) and laser irradiation induce photooxidation [J].