The Agrobacterium DNA transfer complex

被引:77
作者
Zupan, J
Zambryski, P
机构
关键词
D O I
10.1080/713608150
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Agrobacterium tumefaciens elicits tumorous growths on plants by transforming plant cells with a segment of its own DNA. This trait led to development of Agrobacterium as a vector for genetic transformation of flowering plants. The transformation process is a unique mixture of several distinct steps, some of which are evolutionarily and functionally related to bacterial conjugation, and some of which converge with eukaryotic cellular processes. Recent work has advanced our understanding of each of these steps. The early reactions in the production of an ssDNA transfer intermediate (T-strand), mediated by the VirD1/D2 proteins, are chemically similar to formation of a relaxosome in bacterial conjugation. The T-strand is coated by the ssDNA binding protein VirE2; however, whether this binding occurs in the bacterium or in planta is disputed. VirB proteins, related to proteins for the conjugal transfer of DNA between bacteria, most likely form the transfer apparatus. VirD2, which remains covalently bound to the 5' end of the T-strand, and VirE2 both localize to the nucleus of plant cells. VirE2 also mediates the nuclear accumulation of ssDNA but only when the protein is bound to the ssDNA. Genetically, VirD2 is required for faithful integration of the 5' end and VirE2 for the 3' end of the T-strand. The steps of the process currently under active investigation are the assembly of the export apparatus and the enzymology of integration.
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页码:279 / 295
页数:17
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