Comparison of molecular and conventional methods for typing of enteroviral isolates

被引：8

作者：

Manayani, DJ

Shaji, RV

Fletcher, GJ

Cherian, T

Murali, N

Sathish, N

Solomon, T

Gnanamuthu, C

Sridharan, G ^{[1
]}

机构：

[1] Christian Med Coll & Hosp, Dept Clin Virol, Vellore 632004, Tamil Nadu, India

[2] Christian Med Coll & Hosp, Dept Haematol, Vellore 632004, Tamil Nadu, India

[3] Christian Med Coll & Hosp, Dept Child Hlth, Vellore 632004, Tamil Nadu, India

[4] Christian Med Coll & Hosp, Dept Neurol, Vellore 632004, Tamil Nadu, India

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 03期

关键词：

D O I：

10.1128/JCM.40.3.1069-1070.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Twenty-eight enteroviral isolates obtained from various clinical specimens were typed by Lim-Benyesh-Melnick (LBM) pool-based neutralization, PCR-restriction fragment length polymorphism (RFLP), and partial sequencing of the VP1 region of the enteroviral genome. Sequencing was found to be a good alternative to LBM typing, while PCR-RFLP was inadequate for identification of enteroviral isolates.

引用

页码：1069 / 1070

页数：2

共 13 条

[1] AMPLIFICATION OF RHINOVIRUS SPECIFIC NUCLEIC-ACIDS FROM CLINICAL-SAMPLES USING THE POLYMERASE CHAIN-REACTION [J].