Cloning, expression, and distribution of a Ca2+-activated K+ channel beta-subunit from human brain

被引:143
作者
TsengCrank, J
Godinot, N
Johansen, TE
Ahring, PK
Strobaek, D
Mertz, R
Foster, CD
Olesen, SP
Reinhart, PH
机构
[1] DUKE UNIV,MED CTR,DEPT NEUROBIOL,DURHAM,NC 27710
[2] GLAXO WELLCOME RES INST,RES TRIANGLE PK,NC 27709
[3] NEUROSEARCH,DK-2600 GLOSTRUP,DENMARK
关键词
D O I
10.1073/pnas.93.17.9200
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We have cloned and expressed a Ca2+-activated K+ channel beta-subunit from human brain, The open reading frame encodes a 191-amino acid protein possessing significant homology to a previously described subunit cloned from bovine muscle. The gene for this subunit is located on chromosome 5 at band q34 (hslo-beta), There is no evidence for alternative RNA splicing of this gene product, hslo-beta mRNA is abundantly expressed in smooth muscle, but expression levels are low in most other tissues, including brain, Brain subregions in which beta-subunit mRNA expression is relatively high are the hippocampus and corpus callosum, The coexpression of hslo-beta mRNA together with hslo-alpha subunits in either Xenopus oocytes or stably transfected HEK 293 cells gives rise to Ca2+-activated potassium currents with a much increased calcium and/or voltage sensitivity, These data indicate that the beta-subunit shows a tissue distribution different to that of the alpha-subunit, and in many tissues there may be no association of alpha-subunits with beta-subunits, These beta-subunits can play a functional role in the regulation of neuronal excitability by tuning the Ca2+ and/or the voltage dependence of alpha-subunits.
引用
收藏
页码:9200 / 9205
页数:6
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