Osteogenesis induction of periodontal ligament cells onto bone morphogenic protein-2 immobilized PCL fibers

被引：48

作者：

Kim, Sung Eun ^{[1
,2
]}

Yun, Young-Pil ^{[1
,2
]}

Han, Yun-Kyung ^{[3
]}

Lee, Deok-Won ^{[3
]}

Ohe, Joo-Young ^{[3
]}

Lee, Baek-Soo ^{[3
]}

Song, Hae-Ryong ^{[1
,2
]}

Park, Kyeongsoon ^{[4
]}

Choi, Byung-Joon ^{[3
]}

机构：

[1] Korea Univ, Med Ctr, Guro Hosp, Dept Orthoped Surg, Seoul 152703, South Korea

[2] Korea Univ, Med Ctr, Guro Hosp, Rare Dis Inst, Seoul 152703, South Korea

[3] Kyung Hee Univ, Sch Dent, Dept Oral & Maxillofacial Surg, Seoul 130701, South Korea

[4] Korea Basic Sci Inst, Chuncheon Ctr, Div Bioimaging, Chunchon 200701, Gangwon Do, South Korea

来源：

CARBOHYDRATE POLYMERS | 2014年 / 99卷

关键词：

Periodontal ligament cells (PDLCs); Bone morphogenic protein-2 (BMP-2); PCL fiber; Osteogenesis induction; ALVEOLAR RIDGE AUGMENTATION; SURFACE FUNCTIONALIZATION; IN-VITRO; TITANIUM; TISSUE; REGENERATION; EXPRESSION; SCAFFOLD; IMPLANT; HEPARIN;

D O I：

10.1016/j.carbpol.2013.08.053

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

The purpose of this study was to develop bone morphogenic protein-2 (BMP-2) immobilized PCL fibers to induce osteogenic differentiation of periodontal ligament cells (PDLCs). The PCL fiber surface was modified with heparin-dopamine (Hep-DOPA) (Hep-PCL) and further immobilized with BMP-2 (BMP-2/Hep-PCL). PCL fibers and surface-modified PCL fibers (Hep-PCL and BMP-2/Hep-PCL) were characterized by X-ray photoelectron spectroscopy (XPS) and contact angle. Osteogenic differentiation of PDLCs was demonstrated by alkaline phosphatase (ALP) activity, calcium deposition, and gene expression. The results of XPS and contact angle revealed that Hep-DOPA and BMP-2 were successfully immobilized onto the PCL surface and that the BMP-2/Hep-PCL fibers have more hydrophilic surface properties than PCL fibers alone. ALP activity, calcium deposition, and gene expression on BMP-2/Hep-PCL fibers showed significantly induced osteogenic differentiation relative to PCL fibers. Therefore, we suggest that BMP-2/Hep-PCL fibers have the potential to effectively induce osteogenic differentiation of PDLCs. (C) 2013 Published by Elsevier Ltd.

引用

页码：700 / 709

页数：10

共 54 条

[1]

Application of periodontal ligament cell sheet for periodontal regeneration: a pilot study in beagle dogs [J].