Chloride channel blockers decrease intracellular pH in cultured renal epithelial LLC-PK1 cells

被引：16

作者：

Brown, CDA

Dudley, AJ

机构：

[1] Department of Physiological Sciences, Medical School, University of Newcastle Upon Tyne, Newcastle upon Tyne

来源：

BRITISH JOURNAL OF PHARMACOLOGY | 1996年 / 118卷 / 03期

关键词：

chloride channel blockers; LLC-PK1; cells; intracellular pH;

D O I：

10.1111/j.1476-5381.1996.tb15422.x

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The effects of chloride channel blockers upon intracellular pH (pH(i)) were examined in renal epithelial monolayers of LLC-PK1 cells. A significant intracellular acidification was found with addition of 100 mu M 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB), niflumic acid, flufenamate and diphenylamine-2-carboxylate (DPC) but not with 4,4'-diisothiocyanatostilbene-2-2'disulphonic acid (DIDS). The effects of these agents upon pH(i) was dose-dependent with apparent K-0.5 values of: 16.7+/-0.3 mu M, 34.2+/-0.9 mu M and 740+/-13 mu M for niflumic acid, flufenamate and DPC respectively. The results indicate that at concentrations commonly used to block channel activity these chloride channel blockers have profound effects upon pH(i).

引用

页码：443 / 444

页数：2

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