Antioxidant and antigenotoxic effects of lycopene in obstructive jaundice

被引:29
作者
Aydin, Sevtap [1 ]
Tokac, Mehmet [2 ]
Taner, Gokce [3 ]
Arikok, Ata Turker [4 ]
Dundar, Halit Ziya [2 ]
Ozkardes, Alper Bilal [2 ]
Taslipinar, Mine Yavuz [5 ]
Kilic, Mehmet [6 ]
Basaran, Arif Ahmet [7 ]
Basaran, Nursen [1 ]
机构
[1] Hacettepe Univ, Fac Pharm, Dept Pharmaceut Toxicol, TR-06100 Ankara, Turkey
[2] Etlik Ihtisas Educ & Res Hosp, Dept Surg, Minist Hlth, Ankara, Turkey
[3] Gazi Univ, Fac Sci, Dept Biol, Ankara, Turkey
[4] Diskapi Yildirim Beyazit Educ & Res Hosp, Dept Clin Pathol, Minist Hlth, Ankara, Turkey
[5] Etlik Ihtisas Educ & Res Hosp, Dept Clin Biochem, Minist Hlth, Ankara, Turkey
[6] Univ Yildirim Beyazit, Fac Med, Dept Surg, Ankara, Turkey
[7] Hacettepe Univ, Fac Pharm, Dept Pharmacognosy, TR-06100 Ankara, Turkey
关键词
Lycopene; Cholestasis; Obstructive jaundice; Alkaline single cell gel electrophoresis; DNA damage; Oxidative stress; Peripheral lymphocytes; Liver; Kidney; OXIDATIVE DNA-DAMAGE; BILE-DUCT LIGATION; OXYGEN-FREE RADICALS; LIPID-PEROXIDATION; EXTRAHEPATIC CHOLESTASIS; ISCHEMIA-REPERFUSION; RENAL SUSCEPTIBILITY; BILIARY OBSTRUCTION; HEPATIC-INJURY; BETA-CAROTENE;
D O I
10.1016/j.jss.2012.10.031
中图分类号
R61 [外科手术学];
学科分类号
摘要
Background: Obstructive jaundice, a frequently observed condition caused by obstruction of the common bile duct or its flow and seen in many clinical situations, may end up with serious complications like sepsis, immune depression, coagulopathy, wound breakdown, gastrointestinal hemorrhage, and hepatic and renal failures. Intrahepatic accumulation of reactive oxygen species is thought to be an important cause for the possible mechanisms of the pathogenesis of cholestatic tissue injury from jaundice. Carotenoids have been well described that are able to scavenge reactive oxygen species. Lycopene, a carotenoid present in tomatoes, tomato products, and several fruits and vegetables, have been suggested to have antioxidant activity, so may play a role in certain diseases related to the oxidative stress. The aim of the present study was to determine the effects of lycopene on oxidative stress and DNA damage induced by experimental biliary obstruction in Wistar albino rats. Materials and methods: Daily doses of 100 mg/kg lycopene were given to the bile duct-ligation (BDL) rats orally for 14 days. DNA damage was evaluated by an alkaline comet assay. The levels of aspartate transferase, amino alanine transferase, gamma glutamyl transferase, alkaline phosphatase, and direct bilirubin were analyzed in plasma for the determination of liver functions. The levels of malondialdehyde, reduced glutathione, nitric oxide, catalase, superoxide dismutase, and glutathione S transferase were determined in the liver and kidney tissues. Pro-inflammatory cytokine tumor necrosis factor-alpha level was determined in the liver tissues. Histologic examinations of the liver and kidney tissues were also performed. Results: According to this study, lycopene significantly recovered the parameters of liver functions in plasma, reduced malondialdehyde and nitric oxide levels, enhanced reduced glutathione levels, as well as enhancing all antioxidant enzyme activity in all tissues obtained from the BDL group. Moreover, the parameters of DNA damage in the liver and kidney tissue cells, whole blood cells, and lymphocytes were significantly lower in the lycopene-treated BDL group, compared with the BDL group. Conclusions: Lycopene significantly reduced the DNA damage, and markedly recovered the liver and kidney tissue injuries seen in rats with obstructive jaundice. (C) 2013 Elsevier Inc. All rights reserved.
引用
收藏
页码:285 / 295
页数:11
相关论文
共 77 条
  • [1] Tomato lycopene and low density lipoprotein oxidation: A human dietary intervention study
    Agarwal, S
    Rao, AV
    [J]. LIPIDS, 1998, 33 (10) : 981 - 984
  • [2] THE EFFECT OF VARIOUS ANTIOXIDANTS AND OTHER MODIFYING AGENTS ON OXYGEN-RADICAL-GENERATED DNA-DAMAGE IN HUMAN-LYMPHOCYTES IN THE COMET ASSAY
    ANDERSON, D
    YU, TW
    PHILLIPS, BJ
    SCHMEZER, P
    [J]. MUTATION RESEARCH, 1994, 307 (01): : 261 - 271
  • [3] Expression of inducible nitric oxide synthase in the liver of bile duct-ligated Wistar rats with modulation by lymphomononuclear cells
    Arriero, MD
    López-Farré, A
    Fryeiro, O
    Rodríguez-Feo, JA
    Velasco, S
    García-Durán, M
    Fortes, J
    de la Pinta, JC
    Muñoz, LE
    Celdrán, A
    [J]. SURGERY, 2001, 129 (03) : 255 - 266
  • [4] Gut regulatory peptides bombesin and neurotensin reduce hepatic oxidative stress and histological alterations in bile duct ligated rats
    Assimakopoulos, SF
    Vagianos, CE
    Zervoudakis, G
    Filos, KS
    Georgiou, C
    Nikolopoulou, V
    Scopa, CD
    [J]. REGULATORY PEPTIDES, 2004, 120 (1-3) : 185 - 193
  • [5] Bile duct ligation in rats: A reliable model of hepatorenal syndrome?
    Assimakopoulos, Stelios F.
    Vagianos, Constantine E.
    [J]. WORLD JOURNAL OF GASTROENTEROLOGY, 2009, 15 (01) : 121 - 123
  • [6] Effects of lycopene against cisplatin-induced nephrotoxicity and oxidative stress in rats
    Atessahin, A
    Yilmaz, S
    Karahan, I
    Ceribasi, AO
    Karaoglu, A
    [J]. TOXICOLOGY, 2005, 212 (2-3) : 116 - 123
  • [7] Effect of Rosuvastatin on Cholestasis-Induced Hepatic Injury in Rat Livers
    Awad, Azza S.
    Kamel, Rehab
    [J]. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY, 2010, 24 (02) : 89 - 94
  • [8] BIOCHEMICAL ASPECTS OF FREE-RADICALS
    BASAGA, HS
    [J]. BIOCHEMISTRY AND CELL BIOLOGY, 1990, 68 (7-8) : 989 - 998
  • [9] CYTOKINES TUMOR-NECROSIS-FACTOR AND INTERLEUKIN-6 IN EXPERIMENTAL BILIARY OBSTRUCTION IN MICE
    BEMELMANS, MHA
    GOUMA, DJ
    GREVE, JW
    BUURMAN, WA
    [J]. HEPATOLOGY, 1992, 15 (06) : 1132 - 1136
  • [10] Anti-inflammatory effect of lycopene on carrageenan-induced paw oedema and hepatic ischaemia-reperfusion in the rat
    Bignotto, Leticia
    Rocha, Joao
    Sepodes, Bruno
    Eduardo-Figueira, Maria
    Pinto, Rui
    Chaud, Marco
    de Carvalho, Joao
    Moreno, Heitor, Jr.
    Mota-Filipe, Helder
    [J]. BRITISH JOURNAL OF NUTRITION, 2009, 102 (01) : 126 - 133