Solid phase synthesis of oligodeoxynucleoside phosphorodithioates by a phosphotriester method using a chemoselective coupling reagent

被引：5

作者：

Kehler, J ^{[1
]}

Puschl, A ^{[1
]}

Dahl, O ^{[1
]}

机构：

[1] UNIV COPENHAGEN, HC ORSTED INST, DEPT CHEM, DK-2100 COPENHAGEN, DENMARK

来源：

TETRAHEDRON LETTERS | 1996年 / 37卷 / 44期

关键词：

D O I：

10.1016/0040-4039(96)01820-5

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A phosphotriester method has been developed for solid phase synthesis of oligodeoxynucleoside phosphorodithioates. Couplings are performed by chemoselective oxygen activation of protected nucleoside dithiophosphate anions 1, 7, 8, 9 with 4-nitro-6-trifluoromethylbenzotriazol-1-y-oxy-tris(pyrrolidine)-phosphonium hexafluorophosphate (PyFNOP). Under optimised conditions coupling yields are above 95% for 10 - 20 min couplings, and the products after deblocking are free from phosphorothioate contaminations (detection limit 0.5%). Copyright (C) 1996 Elsevier Science Ltd

引用

页码：8041 / 8044

页数：4

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