Effect of etidronate on bone in orchidectomized and sciatic neurectomized adult rats

被引:36
作者
Iwamoto, J
Takeda, T
Katsumata, T
Tanaka, T
Ichimura, S
Toyama, Y
机构
[1] Keio Univ, Sch Med, Dept Sports Med, Shinjuku Ku, Tokyo 1608582, Japan
[2] Sumitomo Pharmaceut Co Ltd, Res Ctr, Osaka, Japan
[3] Natl Def Med Coll, Dept Orthopaed Surg, Tokorozawa, Saitama 359, Japan
[4] Keio Univ, Sch Med, Dept Orthopaed Surg, Tokyo, Japan
关键词
orchidectomy (ORX); sciatic neurectomy; osteoporosis; etidronate; bone mineral density (BMD); bone resorption;
D O I
10.1016/S8756-3282(01)00687-1
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The purpose of the present study was to determine whether etidronate treatment could prevent bone loss caused by orchidectomy (ORX) and unilateral sciatic neurectomy (NX) in adult male rats. Seventy-four male Wistar rats, aged 10 months, were randomly divided into eight groups: baseline controls (n=10), age-matched sham-operated controls (AMC: n=9), ORX (n=9), NX (n=10), ORX + NX (n=9); ORX + etidronate treatment (ORX + E; n=7); NX + E (n=10), and ORX + NX + E (n=10). Etidronate treatment (10 mg/kg per day subcutaneously) was initiated 2 weeks after surgery and was continued for 2 weeks. Four weeks after surgery, bone mineral density (BMD) of the proximal and middle tibia (PT and MT, respectively), distal and middle femur (DF and MF, respectively), and fourth lumbar vertebral body (LVB) was measured by dual-energy X-ray absorptiometry (Model DCS-600, Aloka, Tokyo, Japan). The mechanical properties of the MF and third LVB were measured by three-point bending and compression tests, respectively. Levels of urinary deoxypyridinoline (Dpd) and serum osteocalcin (Oc) were also measured by enzyme-linked immunosorbent assay. Four weeks of aging had no significant effects on BNID, bone mechanical properties, or bone markers. ORX significantly increased the levels of urinary Dpd and serum Oc, which resulted in significant decreases in BNID of the PT, NIT, DF, MF, and fourth LVB, as well as the mechanical strength (maximum load) of the MF and third LVB. NX significantly increased levels of urinary Dpd and decreased levels of serum Oc, resulting in a significant decrease in BNID of the PT, DF, and fourth LVB. The ORX-induced decrease in BNID of the PT was more pronounced when combined with NX. Etidronate treatment for NX, ORX, and ORX + NX rats significantly decreased levels of urinary Dpd and serum Oc, resulting in complete prevention of loss of BMD and/or bone mechanical strength. The present study demonstrates the efficacy of etidronate treatment for prevention of bone loss caused by testosterone deficiency and immobilization in adult male rats. (Bone 30: 360-367-, 2002) (C) 2002 by Elsevier Science Inc. All rights reserved.
引用
收藏
页码:360 / 367
页数:8
相关论文
共 38 条
  • [1] Intermittent etidronate therapy to prevent corticosteroid-induced osteoporosis
    Adachi, JD
    Bensen, WG
    Brown, J
    Hanley, D
    Hodsman, A
    Josse, R
    Kendler, DL
    Lentle, B
    Olszynski, W
    SteMarie, LG
    Tenenhouse, A
    Chines, AA
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 1997, 337 (06) : 382 - 387
  • [2] Long-term effect of testosterone therapy on bone mineral density in hypogonadal men
    Behre, HM
    Kliesch, S
    Leifke, E
    Link, TM
    Nieschlag, E
    [J]. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1997, 82 (08) : 2386 - 2390
  • [3] Intracortical remodeling in adult rat long bones after fatigue loading
    Bentolila, V
    Boyce, TM
    Fyhrie, DP
    Drumb, R
    Skerry, TM
    Schaffler, MB
    [J]. BONE, 1998, 23 (03) : 275 - 281
  • [4] BERTELLONI S, 1995, J BONE MINER RES, V10, P1488
  • [5] CHAPPARD D, 1991, J BONE MINER RES, V6, P673
  • [6] Erben R. G., 2001, Journal of Musculoskeletal & Neuronal Interactions, V1, P225
  • [7] Androgen deficiency induces high turnover osteopenia in aged male rats: A sequential histomorphometric study
    Erben, RG
    Eberle, J
    Stahr, K
    Goldberg, M
    [J]. JOURNAL OF BONE AND MINERAL RESEARCH, 2000, 15 (06) : 1085 - 1098
  • [8] Treatment of isolated hypogonadotropic hypogonadism effect on bone mineral density and bone turnover
    Guo, CY
    Jones, TH
    Eastell, R
    [J]. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1997, 82 (02) : 658 - 665
  • [9] HUMAN PARATHYROID HORMONE-(1-34) INCREASES BONE MASS IN OVARIECTOMIZED AND ORCHIDECTOMIZED RATS
    HOCK, JM
    GERA, I
    FONSECA, J
    RAISZ, LG
    [J]. ENDOCRINOLOGY, 1988, 122 (06) : 2899 - 2904
  • [10] PROSTAGLANDIN-E2 ENHANCES CORTICAL BONE MASS AND ACTIVATES INTRACORTICAL BONE REMODELING IN INTACT AND OVARIECTOMIZED FEMALE RATS
    JEE, WSS
    MORI, S
    LI, XJ
    CHAN, S
    [J]. BONE, 1990, 11 (04) : 253 - 266