AS1411 aptamer tagged PLGA-lecithin-PEG nanoparticles for tumor cell targeting and drug delivery

被引：204

作者：

Aravind, Athulya ^{[1
]}

Jeyamohan, Prashanti ^{[1
]}

Nair, Remya ^{[1
]}

Veeranarayanan, Srivani ^{[1
]}

Nagaoka, Yutaka ^{[1
]}

Yoshida, Yasuhiko ^{[1
]}

Maekawa, Toru ^{[1
]}

Kumar, D. Sakthi ^{[1
]}

机构：

[1] Toyo Univ, Bio Nano Elect Res Ctr, Grad Sch Interdisciplinary New Sci, Kawagoe, Saitama 3508585, Japan

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2012年 / 109卷 / 11期

关键词：

targeted drug delivery; PLGA nanoparticles; liposomes; AS1411; aptamer; paclitaxel; drug release; POLYMER HYBRID NANOPARTICLES; CONTROLLED-RELEASE; IN-VITRO; PACLITAXEL TAXOL(R); CANCER; NANOSPHERES; DISCOVERY; CURCUMIN; SHELL; CORE;

D O I：

10.1002/bit.24558

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Liposomes and polymers are widely used drug carriers for controlled release since they offer many advantages like increased treatment effectiveness, reduced toxicity and are of biodegradable nature. In this work, anticancer drug-loaded PLGA-lecithin-PEG nanoparticles (NPs) were synthesized and were functionalized with AS1411 anti-nucleolin aptamers for site-specific targeting against tumor cells which over expresses nucleolin receptors. The particles were characterized by transmission electron microscope (TEM) and X-ray photoelectron spectroscopy (XPS). The drug-loading efficiency, encapsulation efficiency and in vitro drug release studies were conducted using UV spectroscopy. Cytotoxicity studies were carried out in two different cancer cell lines, MCF-7 and GI-1 cells and two different normal cells, L929 cells and HMEC cells. Confocal microscopy and flowcytometry confirmed the cellular uptake of particles and targeted drug delivery. The morphology analysis of the NPs proved that the particles were smooth and spherical in shape with a size ranging from 60 to 110?nm. Drug-loading studies indicated that under the same drug loading, the aptamer-targeted NPs show enhanced cancer killing effect compared to the corresponding non-targeted NPs. In addition, the PLGA-lecithin-PEG NPs exhibited high encapsulation efficiency and superior sustained drug release than the drug loaded in plain PLGA NPs. The results confirmed that AS1411 aptamer-PLGA-lecithin-PEG NPs are potential carrier candidates for differential targeted drug delivery. Biotechnol. Bioeng. 2012; 109: 29202931. (c) 2012 Wiley Periodicals, Inc.

引用

页码：2920 / 2931

页数：12

共 34 条

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