A DNA duplex with extremely enhanced thermal stability based on controlled immobilization on gold nanoparticles

被引：48

作者：

Akamatsu, K

Kimura, M

Shibata, Y

Nakano, S

Miyoshi, D

Nawafune, H

Sugimoto, N

机构：

[1] Konan Univ, Frontier Inst Biomol Engn Res, Dept Chem, Fac Sci & Engn, Kobe, Hyogo 6588501, Japan

[2] Konan Univ, Grad Sch Sci, Kobe, Hyogo 6588501, Japan

来源：

NANO LETTERS | 2006年 / 6卷 / 03期

关键词：

D O I：

10.1021/nl0524748

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The effect of DNA loadings on the thermal stability of DNA duplex immobilized on gold nanoparticles has been investigated. The modestly loaded duplexes on the gold nanoparticles showed enhanced thermal stability, as compared to that of the free duplex (without gold nanoparticles). However, the highly loaded duplex showed stability similar to that of free duplex. The stability could be controlled over a wide temperature range simply by varying the salt concentration (over 50 degrees C). Additionally, the gold nanoparticles with modestly loaded oligonucleotides could be used as nanoprobes for effective and fast strand exchange reactions, based on the increased thermal stability of the immobilized duplex. These results indicate that the interaction between the duplex and the nanoparticle surface plays an important role in determining the stability of the duplex.

引用

收藏

页码：491 / 495

页数：5

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