Detection of trinitrotoluene (TNT) extracted from soil using a surface plasmon resonance (SPR)-based sensor platform

被引：10

作者：

Strong, AA ^{[1
]}

Stimpson, DI ^{[1
]}

Bartholomew, DU ^{[1
]}

Jenkins, TF ^{[1
]}

Elkind, JL ^{[1
]}

机构：

[1] Texas Instruments Inc, Dallas, TX 75265 USA

来源：

DETECTION AND REMEDIATION TECHNOLOGIES FOR MINES AND MINELIKE TARGETS IV, PTS 1 AND 2 | 1999年 / 3710卷

关键词：

biosensor; land mines; TNT; trinitrotoluene; SPR; surface plasmon resonance;

D O I：

10.1117/12.357059

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

An antibody-based competition assay has been developed using a surface plasmon resonance (SPR) sensor platform for the detection of trinitrotoluene (TNT) in soil extract solutions. The objective of this work is to develop a sensor-based assay technology to use in the field for real-time detection of land mines. This immunoassay combines very simple bio-film attachment procedures and a low-cost SPR sensor design to detect TNT in soil extracts. The active bio-surface is a coating of bovine serum albumin that has been decorated with trinitrobenzene groups (TNB-BSA). A blind study on extracts from a large soil matrix was recently performed and results from this study will be presented. These will include demonstrated sensitivity to 1ppm TNT, in a variety of soils, with no false negatives. Potential interferants studied included 2,4-dinitrophenol, 2,4-dinitrotoluene, ammonium nitrate, and 2,4-dichlorophenoxyacetic acid. Cross-reactivity with dinitrotoluene will be discussed. Also, plans to reach sensitivity levels of 1ppb TNT in soil will be described.

引用

页码：362 / 372

页数：11

共 15 条

[1] ELKIND JL, UNPUB SENSORS ACTU B
[2] An electrostatic particle sampler and chemical sensor system for landmine detection by chemical signature
Fisher, M
Cumming, C
la Grone, M
Taylor, R
[J]. DETECTION AND REMEDIATION TECHNOLOGIES FOR MINES AND MINELIKE TARGETS III, PTS 1 AND 2, 1998, 3392 : 565 - 574
[3] DEVELOPMENT OF FIELD SCREENING METHODS FOR TNT, 2,4-DNT AND RDX IN SOIL
JENKINS, TF
WALSH, ME
[J]. TALANTA, 1992, 39 (04) : 419 - 428
[4] COMPARISON OF EXTRACTION TECHNIQUES FOR MUNITIONS RESIDUES IN SOIL
JENKINS, TF
GRANT, CL
[J]. ANALYTICAL CHEMISTRY, 1987, 59 (09) : 1326 - 1331
[5] JONSSON U, 1991, BIOTECHNIQUES, V11, P620
[6] MELENDEZ J, 1996, SENSOR ACTUAT B-CHEM, V35, P1
[7] O'Shannessy D J, 1994, Curr Opin Biotechnol, V5, P65, DOI 10.1016/S0958-1669(05)80072-2
[8] Phelan J. M., 1997, SAND971426
[9] Rabbany Sina Y., 1994, Critical Reviews in Biomedical Engineering, V22, P307
[10] Rouhi AM, 1997, CHEM ENG NEWS, V75, P14, DOI 10.1021/cen-v075n010.p014

← 1 2 →