Genetic analysis of the mouse brain proteome

被引：189

作者：

Klose, J

Nock, C

Herrmann, M

Stühler, K

Marcus, K

Blüggel, M

Krause, E

Schalkwyk, LC

Rastan, S

Brown, SDM

Büssow, K

Himmelbauer, H

Lehrach, H

机构：

[1] Humboldt Univ, Charite, Inst Humangenet, D-13353 Berlin, Germany

[2] Free Univ Berlin, Fachbereich Biol, D-14195 Berlin, Germany

[3] Max Planck Inst Mol Genet, D-14195 Berlin, Germany

[4] Ruhr Univ Bochum, Inst Physiol Chem, D-44780 Bochum, Germany

[5] Protagen AG, D-44801 Bochum, Germany

[6] Inst Mol Pharmakol, D-13125 Berlin, Germany

[7] Inst Psychiat, SGDP Res Ctr, London SE5 8AF, England

[8] MWB Business Exchange, Cambridge CB1 1BH, England

[9] MRC, Mammalian Genet Unit, Oxford OX11 0RD, England

[10] UK Mouse Genome Ctr, Oxford OX11 0RD, England

来源：

NATURE GENETICS | 2002年 / 30卷 / 04期

关键词：

D O I：

10.1038/ng861

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Proteome analysis is a fundamental step in systematic functional genomics. Here we have resolved 8,767 proteins from the mouse brain proteome by large-gel two-dimensional electrophoresis. We detected 1,324 polymorphic proteins from the European collaborative interspecific backcross. Of these, we mapped 665 proteins genetically and identified 466 proteins by mass spectrometry. Qualitatively polymorphic proteins, to 96%, reflect changes in conformation and/or mass. Quantitatively polymorphic proteins show a high frequency (73%) of allele-specific transmission in codominant heterozygotes. Variations in protein isoforms and protein quantity often mapped to chromosomal positions different from that of the structural gene, indicating that single proteins may act as polygenic traits. Genetic analysis of proteomes may detect the types of polymorphism that are most relevant in disease-association studies.

引用

页码：385 / 393

页数：9

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