Super agonist actions of clothianidin and related compounds on the SADβ2 nicotinic acetylcholine receptor expressed in Xenopus laevis oocytes

被引：47

作者：

Ihara, M

Matsuda, K ^{[1
]}

Shimomura, M

Sattelle, DB

Komai, K

机构：

[1] Kinki Univ, Grad Sch Agr, Nara 6318505, Japan

[2] Univ Oxford, Dept Human Anat & Genet, MRC, Funct Genet Unit, Oxford OX1 3QX, England

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2004年 / 68卷 / 03期

关键词：

clothianidin; Drosophila melanogaster; SAD-chicken beta 2 hybrid nicotinic receptor; imidacloprid; neonicotinoid; nicotinic acetylcholine receptor;

D O I：

10.1271/bbb.68.761

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To compare the actions of clothianidin, a neonicotinoid acting on insect nicotinic acetylcholine receptors, and related compounds with that of imidacloprid, the compounds were tested on the Drosophila SAD-chicken beta2 nicotinic acetylcholine receptor expressed in Xenopus laevis oocytes using two-electrode voltage-clamp electrophysiology. The maximum response of the SADbeta2 nicotinic receptor to clothianidin was larger than that observed for acetylcholine. Ring breakage of the imidazolidine ring of imidacloprid resulting in the generation of a guanidine group was critical for this super agonist action.

引用

页码：761 / 763

页数：3

共 12 条

[1] PHYSIOLOGICAL-PROPERTIES OF NEURONAL NICOTINIC RECEPTORS RECONSTITUTED FROM THE VERTEBRATE BETA-2 SUBUNIT AND DROSOPHILA ALPHA-SUBUNITS [J].