Surface plasmon resonance characterization of photoswitchable antigen-antibody interactions

被引：36

作者：

Kaganer, E

Pogreb, R

Davidov, D

Willner, I ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Inst Chem, IL-91904 Jerusalem, Israel

[2] Coll Judea & Samaria, Inst Res, IL-44837 Ariel, Israel

来源：

LANGMUIR | 1999年 / 15卷 / 11期

关键词：

D O I：

10.1021/la981147v

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Photostimulated association and dissociation of anti-dinitrophenyl antibody, anti-DNP-Ab, to and from a dinitrospiropyran photoisomerizable monolayer is probed by surface plasmon resonance (SPR). The anti-DNP-Ab binds to a dinitrospiropyran antigen monolayer, whereas the DNP-AI, dissociates from the protonated dinitromerocyanine monolayer. Reversible binding and dissociation of anti-DNP-Ab to and from the photoisomerizable monolayer was assayed by SPR spectroscopy. Analysis of the SPR data indicates that the average thickness of the antibody layer is ca. 70 Angstrom which corresponds to a surface coverage of ca. 4.4 x 10(-9) mol.cm(-2).

引用

页码：3920 / 3923

页数：4

共 38 条

[1] THIN LANGMUIR-BLODGETT-FILMS STUDIED USING SURFACE PLASMON-POLARITONS [J].