Cloning and functional expression of a human Ca2+-permeable cation channel activated by calcium store depletion

被引：339

作者：

Zitt, C ^{[1
]}

Zobel, A ^{[1
]}

Obukhov, AG ^{[1
]}

Harteneck, C ^{[1
]}

Kalkbrenner, F ^{[1
]}

Luckhoff, A ^{[1
]}

Schultz, G ^{[1
]}

机构：

[1] FREE UNIV BERLIN,INST PHARMAKOL,D-14195 BERLIN,GERMANY

来源：

NEURON | 1996年 / 16卷 / 06期

关键词：

D O I：

10.1016/S0896-6273(00)80145-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Depletion of intracellular calcium stores generates a signal that activates Ca2+-permeable channels in the plasma membrane. We have identified a human cDNA, TRPC1A, from a human fetal brain cDNA library. TRPC1A is homologous to the cation channels trp and trpl in Drosophila and is a splice variant of the recently identified cDNA Htrp-1. Expression of TRPC1A in CHO cells induced nonselective cation currents with similar permeabilities for Na+, Ca2+, and Cs+. The currents were activated by intracellular infusion of myo inositol-1,4,5-trisphosphate or thapsigargin. Expression of TRPC1A significantly enhanced increases in the intracellular free calcium concentration induced by Ca2+ restitution after prolonged depletion. Similar results were obtained in Sf9 cells. We conclude that TRPC1A encodes a Ca2+-permeable cation channel activated by depletion of intracellular calcium stores.

引用

页码：1189 / 1196

页数：8

共 26 条

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