Particle Size, Surface Coating, and PEGylation Influence the Biodistribution of Quantum Dots in Living Mice

被引：379

作者：

Schipper, Meike L. ^{[1
,2
]}

Iyer, Gopal ^{[3
,4
]}

Koh, Ai Leen ^{[5
]}

Cheng, Zhen ^{[1
,2
]}

Ebenstein, Yuval ^{[3
,4
]}

Aharoni, Assaf ^{[8
,9
]}

Keren, Shay ^{[1
,2
]}

Bentolila, Laurent A. ^{[3
,4
]}

Li, Jianquing ^{[3
,4
]}

Rao, Jianghong ^{[1
,2
]}

Chen, Xiaoyuan ^{[1
,2
]}

Banin, Uri ^{[8
,9
]}

Wu, Anna M. ^{[6
,7
]}

Sinclair, Robert ^{[5
]}

Weiss, Shimon ^{[3
,4
]}

Gambhir, Sanjiv S. ^{[1
,2
]}

机构：

[1] Stanford Univ, Dept Radiol, Bio X Program, MIPS, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Bioengn, Bio X Program, MIPS, Stanford, CA 94305 USA

[3] Univ Calif Los Angeles, Sch Med, CNSI, Los Angeles, CA 90005 USA

[4] Univ Calif Los Angeles, Sch Med, Dept Chem & Biochem, Los Angeles, CA 90005 USA

[5] Stanford Univ, Dept Mat Sci & Engn, Stanford Nanocharacterizat Lab, Stanford, CA 94305 USA

[6] Univ Calif Los Angeles, Sch Med, Crump Inst Mol Imaging, Los Angeles, CA 90005 USA

[7] Univ Calif Los Angeles, Sch Med, Dept Mol & Med Pharmacol, Los Angeles, CA 90005 USA

[8] Hebrew Univ Jerusalem, Dept Phys Chem, IL-91904 Jerusalem, Israel

[9] Hebrew Univ Jerusalem, Ctr Nanosci & Nanotechnol, IL-91904 Jerusalem, Israel

来源：

SMALL | 2009年 / 5卷 / 01期

关键词：

biodistribution; imaging; nanoparticles; quantum dots; tomography; POLYETHYLENE-GLYCOL-LIPOSOMES; NEAR-INFRARED FLUORESCENCE; IN-VIVO; PHARMACOKINETICS; CLEARANCE; CELLS; NANOPARTICLES; NANOCRYSTALS; OPSONIZATION; PERFORMANCE;

D O I：

10.1002/smll.200800003

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This study evaluates the influence of particle size, PEGylation, and surface coating on the quantitative biodistribution of near-infrared-emitting quantum dots (QDs) in mice. Polymer- or peptide-coated Cu-64-labeled QDs 2 or 12 nm in diameter, with or without polyethylene glycol (PEG) of molecular weight 2000, are studied by serial micropositron emission tomography imaging and region-of-interest analysis, as well as transmission electron microscopy and inductively coupled plasma mass spectrometry. PEGylation and peptide coating slow QD uptake into the organs of the reticuloendothelial system (RES), liver and spleen, by a factor of 6-9 and 2-3, respectively. Small particles are in part renally excreted. Peptide-coated particles are cleared from liver faster than physical decay alone would suggest. Renal excretion of small QDs and slowing of RES clearance by PEGylation or peptide surface coating are encouraging steps toward the use of modified QDs for imaging living subjects.

引用

页码：126 / 134

页数：9

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