Chain fluidity and phase behaviour of phospholipids as revealed by FTIR and sum-frequency spectroscopy

被引:12
作者
Löbau, J
Sass, M
Pohle, W
Selle, C
Koch, MHJ
Wolfrum, K
机构
[1] Phys Tech Bundesanstalt, Fachlab 523, D-38116 Braunschweig, Germany
[2] Tech Univ Munich, Dept Phys E11, D-85748 Garching, Germany
[3] Univ Jena, Inst Mol Biol, D-07745 Jena, Germany
[4] DESY, EMBL Outstn, D-20603 Hamburg, Germany
[5] FH Karlsruhe, FB Elekt Energietech, D-76133 Karlsruhe, Germany
关键词
phospholipids; FTIR spectroscopy; sum-frequency spectroscopy; hydration; surface chemistry;
D O I
10.1016/S0022-2860(98)00714-5
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
The functioning of biological membranes seems to require a certain degree of fluidity of their components. The fluidity of the lipids forming the matrix of such membranes is related to the order/disorder state of their hydrocarbon chains. In this study, vibrational spectroscopy is applied to probe the chain conformation (as determining the order) of a number of phospholipids with varying intrinsic fluidities as a function of water activity (hydration). Using conventional Fourier-transform infrared (FTIR) spectroscopy and sum-frequency spectroscopy (SFS) enables one to characterize and, thus, to compare physical properties of the molecules in the bulk and in the superficial layer of a specimen, respectively. The results demonstrate the ability of FTIR spectroscopy not only to classify the lipids with respect to chain ordering, but also to detect lyotropic (hydration-driven) phase transitions. It could be shown that the main transition of mixed-chain oleoyl/palmitoyl phosphatidylcholines (POPC, OPPC) occurs at room temperature and a defined water activity of the films investigated, as also confirmed by small-angle X-ray scattering (SAXS). Equivalent effects were found for POPC in appropriately designed SFS experiments thus evidencing lipid phase transitions by this method for the first time. This opens up a new avenue to elucidate basic aspects of lipid phase behaviour using single bilayer membranes as models of the in vivo state. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:407 / 411
页数:5
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