SufC hydrolyzes ATP and interacts with SufB from Thermotoga maritima

被引：52

作者：

Rangachari, K ^{[1
]}

Davis, CT ^{[1
]}

Eccleston, JF ^{[1
]}

Hirst, EMA ^{[1
]}

Saldanha, JW ^{[1
]}

Strath, M ^{[1
]}

Wilson, RJM ^{[1
]}

机构：

[1] Natl Inst Med Res, London NW7 1AA, England

来源：

FEBS LETTERS | 2002年 / 514卷 / 2-3期

关键词：

ABC-transporter; ATPasc; suf operon; Thermotoga maritima; Escherichia coli;

D O I：

10.1016/S0014-5793(02)02369-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genetic experiments in bacteria have shown the suf operon is involved in iron homeostasis and the oxidative stress response. The sufB and sufC genes that always occur together in bacteria are also found in plants, and even the malaria parasite, associated with the plastid organelle. Although the suf operon is believed to encode an iron-dependent ABC-transporter there is no direct evidence. By immunolocalization we show here that SufB and SufC are associated with the membrane of Escherichia coli. We also present kinetic studies with a recombinant version of SufC from Thermotoga maritima that shows it is an ATPase and that it interacts with SufB in vitro. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：225 / 228

页数：4

共 19 条

[1] Protein import into chloroplasts [J].