Silencing of the MT1-MMP/G6PT axis suppresses calcium mobilization by sphingosine-1-phosphate in glioblastoma cells

被引：23

作者：

Fortier, Simon ^{[1
]}

Labelle, Dominique ^{[2
]}

Sina, Asmaa ^{[1
]}

Moreau, Robert ^{[2
]}

Annabi, Borhane ^{[1
]}

机构：

[1] Univ Quebec, Dept Chim, Ctr Biomed, Oncol Mol Lab, Montreal, PQ H3C 3P8, Canada

[2] Univ Quebec, Ctr Biomed, Dept Sci Biol, Lab Metab Osseux, Montreal, PQ H3C 3P8, Canada

来源：

FEBS LETTERS | 2008年 / 582卷 / 05期

基金：

加拿大自然科学与工程研究理事会;

关键词：

MT1-MMP; S1P; glucose-6-phosphate transporter; calcium mobilization; cell migration;

D O I：

10.1016/j.febslet.2008.01.061

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The contributions of membrane type-1 matrix metalloproteinase (MT1-MMP) and of the glucose-6-phosphate transporter (G6PT) in sphingosine-1-pbosphate (SIP)-mediated Ca2+ mobilization were assessed in glioblastoma cells. We show that gene silencing of MT1-MMP or G6PT decreased the extent of SIP-induced Ca2+ mobilization, chemotaxis, and extracellular signal-related kinase phosphorylation. Chlorogenic acid and (-)-epigallocatechin-3-gallate, two diet-derived inhibitors of G6PT and of MT1-MMP, respectively, reduced SIP-mediated Ca2+ mobilization. An intact MT1-MMP/G6PT signaling axis is thus required for efficient Ca2+ mobilization in response to bioactive lipids such as SIP. Targeted inhibition of either MT1-MMP or G6PT may lead to reduced infiltrative and invasive properties of brain tumor cells. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：799 / 804

页数：6

共 40 条

[1]

Probing the infiltrating character of brain tumors:: inhibition of RhoA/ROK-mediated CD44 cell surface shedding from glioma cells by the green tea catechin EGCg [J].

Annabi, B ;

Bouzeghrane, M ;

Moumdjian, R ;

Moghrabi, A ;

Béliveau, R .

JOURNAL OF NEUROCHEMISTRY, 2005, 94 (04) :906-916

[2]

Hyaluronan cell surface binding is induced by type I collagen and regulated by caveolae in glioma cells [J].

Annabi, B ;

Thibeault, S ;

Moumdjian, R ;

Béliveau, R .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (21) :21888-21896

[3]

Matrix metalloproteinase regulation of sphingosine-1-phosphate-induced angiogenic properties of bone marrow stromal cells [J].

Annabi, B ;

Thibeault, S ;

Lee, YT ;

Bousquet-Gagnon, N ;

Eliopoulos, N ;

Barrette, S ;

Galipeau, J ;

Béliveau, R .

EXPERIMENTAL HEMATOLOGY, 2003, 31 (07) :640-649

[4]

Green tea polyphenol (-)-epigallocatechin 3-gallate inhibits MMP-2 secretion and MT1-MMP-driven migration in glioblastoma cells [J].

Annabi, B ;

Lachambre, MP ;

Bousquet-Gagnon, N ;

Pagé, M ;

Gingras, D ;

Béliveau, R .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2002, 1542 (1-3) :209-220

[5]

Necrosis induction in glioblastoma cells reveals a new "bioswitch'' function for the MT1-MMP/G6PT signaling axis in proMMP-2 activation versus cell death decision [J].

Belkaid, Anissa ;

Fortier, Simon ;

Cao, Jian ;

Annabi, Borhane .

NEOPLASIA, 2007, 9 (04) :332-340

[6]

The chemopreventive properties of chlorogenic acid reveal a potential new role for the microsomal glucose-6-phosphate translocase in brain tumor progression [J].

Belkaid, Anissa ;

Currie, Jean-Christophe ;

Desgagnes, Julie ;

Annabi, Borhane .

CANCER CELL INTERNATIONAL, 2006, 6 (1)

[7]

Silencing of the human microsomal glucose-6-phosphate translocase induces glioma cell death: Potential new anticancer target for curcumin [J].

Belkaid, Anissa ;

Copland, Ian B. ;

Massillon, Duna ;

Annabi, Borhane .

FEBS LETTERS, 2006, 580 (15) :3746-3752

[8]

Metabolic profiling of cell growth and death in cancer: applications in drug discovery [J].

Boros, LG ;

Cascante, M ;

Lee, WNP .

DRUG DISCOVERY TODAY, 2002, 7 (06) :364-372

[9]

Expression and localization of MT1-MMP and furin in the glomerular wall of short- and long-term diabetic rats [J].

Boucher, E ;

Mayer, G ;

Londono, I ;

Bendayan, M .

KIDNEY INTERNATIONAL, 2006, 69 (09) :1570-1577

[10]

Evolutionary chemistry approach toward finding novel inhibitors of the type 2 diabetes target glucose-6-phosphate translocase [J].

Bräuer, S ;

Almstetter, M ;

Antuch, W ;

Behnke, D ;

Taube, R ;

Furer, P ;

Hess, S .

JOURNAL OF COMBINATORIAL CHEMISTRY, 2005, 7 (02) :218-226

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