Transfer of hydroxycinnamoyl residues to microsomal proteins from parsley

Microsomal membrane preparations from parsley cells in culture produced polymeric material from [C-14]-feruloyl- and [C-14]-p-coumaroyl-CoA. This reaction could not be inhibited by addition of catalase, suggesting that polymerization did not involve peroxidases and H2O2. About half the labelled polymer could be extracted with aqueous phenol, precipitated and split by trypsin, indicating a protein nature. The labelled proteins appeared as four major discrete bands in SDS-polyacrylamide gels. One of these bands was diminished whereas an additional band became visible after treatment with either N-glycosidase F or endoglycosidase H. Thus, we assume that part of the labelled proteins is N-glycosylated. Copyright (C) 1996 Elsevier Science Ltd