Degradation of Sphingoid Long-Chain Base 1-Phosphates (LCB-1Ps): Functional Characterization and Expression of AtDPL1 Encoding LCB-1P Lyase Involved in the Dehydration Stress Response in Arabidopsis

被引：34

作者：

Nishikawa, Masahiro ^{[1
]}

Hosokawa, Kenta ^{[1
]}

Ishiguro, Mai ^{[1
]}

Minamioka, Hiroki ^{[1
]}

Tamura, Kentaro ^{[2
]}

Hara-Nishimura, Ikuko ^{[2
]}

Takahashi, Yohei ^{[3
]}

Shimazaki, Ken-ichiro ^{[3
]}

Imai, Hiroyuki ^{[1
]}

机构：

[1] Konan Univ, Grad Sch Nat Sci, Dept Biol, Kobe, Hyogo 6588501, Japan

[2] Kyoto Univ, Grad Sch Sci, Dept Bot, Kyoto 6068502, Japan

[3] Kyushu Univ, Fac Sci, Dept Biol, Fukuoka 8108560, Japan

来源：

PLANT AND CELL PHYSIOLOGY | 2008年 / 49卷 / 11期

关键词：

D O I：

10.1093/pcp/pcn149

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Sphingoid long-chain base (LCB) 1-phosphates are degradated by LCB 1-phosphate lyase to C(16) fatty aldehydes and phosphoethanolamine. Here, we confirmed that the At1g27980 gene product, AtDPL1, is a functional LCB-1-phosphate lyase. Expression of green fluorescent protein fusion products in suspension-cultured Arabidopsis cells showed that AtDPL1 is located to the endoplasmic reticulum. The rates of fresh weight decreases of dpl1-1 and dpl1-2 mutants were significantly slower than those of the wild-type plants. This ability to limit their transpiration reflected the leaf temperature of the mutant plants more than that of wild-type plants, suggesting that AtDPL1 plays a role in dehydration stress.

引用

页码：1758 / 1763

页数：6

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