Inhibition of cellulase activity by sunflower polyphenols

被引：22

作者：

Sineiro, J ^{[1
]}

Dominguez, H ^{[1
]}

Nunez, MJ ^{[1
]}

Lema, JM ^{[1
]}

机构：

[1] UNIV VIGO,FAC CIENCIAS OURENSE,DEPT CHEM ENGN,AS LAGOAS 32004,OURENSE,SPAIN

来源：

BIOTECHNOLOGY LETTERS | 1997年 / 19卷 / 06期

关键词：

D O I：

10.1023/A:1018377001557

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The cellulase-inhibitor binding constant (beta) for chlorogenic, ferulic, caffeic and sinapic acids, when these compounds were present in the range of concentrations found during the aqueous extraction of sunflower oil, was calculated. Chlorogenic acid presented a weak inhibiting power (beta = 0.14 L/mmol), whereas sinapic acid conducted as the strongest inhibitor (beta = 14.60 L/mmol). The inhibition effects were not additive, since in the presence of mixtures the enzyme retained 75-85% of its activity.

引用

页码：521 / 524

页数：4

共 14 条

[1]

AKIN DE, 1983, WOOD AGR RESIDUES, P34

[2] OPTIMIZING CONDITIONS FOR ENZYMATIC EXTRACTION OF SUNFLOWER OIL [J].

BADR, FH ;

SITOHY, MZ .

GRASAS Y ACEITES, 1992, 43 (05) :281-283

[3]

BOLANOS G, 1989, GRASAS ACEITES, V40, P146

[4] ENZYMATIC PRETREATMENT TO ENHANCE OIL EXTRACTION FROM FRUITS AND OILSEEDS - A REVIEW [J].

DOMINGUEZ, H ;

NUNEZ, MJ ;

LEMA, JM .

FOOD CHEMISTRY, 1994, 49 (03) :271-286

[5] Enzymatic treatment of sunflower kernels before oil extraction [J].

Dominguez, H ;

Sineiro, J ;

Nunez, MJ ;

Lema, JM .

FOOD RESEARCH INTERNATIONAL, 1995, 28 (06) :537-545

[6]

FAN LT, 1987, CELLULOSE HYDROLYSIS, P76

[7]

FERCHAK JD, 1983, BIOTECHNOL BIOENG, V25, P2865, DOI 10.1002/bit.260251205

[8] THE USE OF ENZYMES IN THE PROCESSING OF OILSEEDS [J].

FULLBROOK, PD .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1983, 60 (02) :476-478

[9] A THEORETICAL-ANALYSIS OF CELLULASE PRODUCT INHIBITION - EFFECT OF CELLULASE BINDING CONSTANT, ENZYME SUBSTRATE RATIO, AND BETA-GLUCOSIDASE ACTIVITY ON THE INHIBITION PATTERN [J].

GUSAKOV, AV ;

SINITSYN, AP .

BIOTECHNOLOGY AND BIOENGINEERING, 1992, 40 (06) :663-671

[10] INHIBITION OF TRICHODERMA-REESEI CELLULASE BY SUGARS AND SOLVENTS [J].

HOLTZAPPLE, M ;

COGNATA, M ;

SHU, Y ;

HENDRICKSON, C .

BIOTECHNOLOGY AND BIOENGINEERING, 1990, 36 (03) :275-287

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