Nonenzymatic RNA Ligation in Water

被引：37

作者：

Pino, Samanta ^{[2
]}

Ciciriello, Fabiana ^{[2
]}

Costanzo, Giovanna ^{[3
]}

Di Mauro, Ernesto ^{[1
]}

机构：

[1] Univ Roma La Sapienza, Dipartimento Genet & Biol Mol, Fdn Ist Pasteur Fdn Cenci Bolognetti, I-00185 Rome, Italy

[2] Univ Roma La Sapienza, Dipartimento Genet & Biol Mol, I-00185 Rome, Italy

[3] CNR, Ist Biol & Patol Mol, I-00185 Rome, Italy

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2008年 / 283卷 / 52期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1074/jbc.M805333200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the nonenzymatic ligation of RNA oligomers in water. Dimers and tetramers are formed in a time-, pH-, and temperature-dependent reaction. Ligation efficiency depends on oligonucleotide length and sequence and is strongly enhanced by adenine-based nucleotide cofactors. Ligation of short RNA fragments could have liberated the prebiotic polymerization systems from the thermodynamically demanding task of reaching a (pre) genetically meaningful size by stepwise addition of one precursor monomer at the time.

引用

页码：36494 / 36503

页数：10

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