PHYL acts to down-regulate TTK88, a transcriptional repressor of neuronal cell fates, by a SINA-dependent mechanism

被引：197

作者：

Tang, AH ^{[1
]}

Neufeld, TP ^{[1
]}

Kwan, E ^{[1
]}

Rubin, GM ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY,HOWARD HUGHES MED INST,DEPT MOL & CELL BIOL,BERKELEY,CA 94720

来源：

CELL | 1997年 / 90卷 / 03期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0092-8674(00)80506-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We show that Tramtrack (TTK88) expression represses neuronal fate determination in the developing Drosophila eye. Phyllopod (PHYL) acts to antagonize this repression by a mechanism that requires Seven In Absentia (SINA) and is associated with decreased TTK88 protein levels, but not reduced ttk88 gene transcription or mRNA stability. We present evidence that SINA, PHYL, and TTK88 physically interact and that SINA interacts genetically and physically with UBCD1, a component of the ubiquitin-dependent protein degradation pathway. Our results suggest a model in which activation of the Sevenless receptor tyrosine kinase induces PHYL expression, which then acts with SINA to target the transcriptional repressor TTK88 for degradation, thereby promoting R7 cell fate specification.

引用

页码：459 / 467

页数：9

共 65 条

[1] Isolation of 10 differentially expressed cDNAs in p53-induced apoptosis: Activation of the vertebrate homologue of the Drosophila seven in absentia gene [J].

Amson, RB ;

Nemani, M ;

Roperch, JP ;

Israeli, D ;

Bougueleret, L ;

LeGall, I ;

Medhioub, M ;

LinaresCruz, G ;

Lethrosne, F ;

Pasturaud, P ;

Piouffre, L ;

Prieur, S ;

Susini, L ;

Alvaro, V ;

Millasseau, P ;

Guidicelli, C ;

Bui, H ;

Massart, C ;

Cazes, L ;

Dufour, F ;

BruzzoniGiovanelli, H ;

Owadi, H ;

Hennion, C ;

Charpak, G ;

Dausset, J ;

Calvo, F ;

Oren, M ;

Cohen, D ;

Telerman, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (09) :3953-3957

[2] NOTCH SIGNALING [J].

ARTAVANISTSAKONAS, S ;

MATSUNO, K ;

FORTINI, ME .

SCIENCE, 1995, 268 (5208) :225-232

[3] THE SPATIAL AND TEMPORAL EXPRESSION PATTERN OF SEVENLESS IS EXCLUSIVELY CONTROLLED BY GENE-INTERNAL ELEMENTS [J].

BASLER, K ;

SIEGRIST, P ;

HAFEN, E .

EMBO JOURNAL, 1989, 8 (08) :2381-2386

[4] REGULATION OF THE COMPLEX PATTERN OF SEVENLESS EXPRESSION IN THE DEVELOPING DROSOPHILA EYE [J].

BOWTELL, DDL ;

KIMMEL, BE ;

SIMON, MA ;

RUBIN, GM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (16) :6245-6249

[5] REPRESSION OF THE DROSOPHILA-FUSHI-TARAZU (FTZ) SEGMENTATION GENE [J].

BROWN, JL ;

SONODA, S ;

UEDA, H ;

SCOTT, MP ;

WU, C .

EMBO JOURNAL, 1991, 10 (03) :665-674

[6]

BROWN JL, 1993, DEVELOPMENT, V117, P45

[7] A GAIN-OF-FUNCTION MUTATION IN DROSOPHILA MAP KINASE ACTIVATES MULTIPLE RECEPTOR TYROSINE KINASE SIGNALING PATHWAYS [J].

BRUNNER, D ;

OELLERS, N ;

SZABAD, J ;

BIGGS, WH ;

ZIPURSKY, SL ;

HAFEN, E .

CELL, 1994, 76 (05) :875-888

[8] CHARACTERIZATION AND USE OF THE DROSOPHILA METALLOTHIONEIN PROMOTER IN CULTURED DROSOPHILA-MELANOGASTER CELLS [J].

BUNCH, TA ;

GRINBLAT, Y ;

GOLDSTEIN, LSB .

NUCLEIC ACIDS RESEARCH, 1988, 16 (03) :1043-1061

[9] Numb diverts notch pathway off the Tramtrack [J].

CamposOrtega, JA .

NEURON, 1996, 17 (01) :1-4

[10] 7 IN ABSENTIA, A GENE REQUIRED FOR SPECIFICATION OF R7 CELL FATE IN THE DROSOPHILA EYE [J].

CARTHEW, RW ;

RUBIN, GM .

CELL, 1990, 63 (03) :561-577

← 1 2 3 4 5 6 7 →