RNAi as a tool for understanding germline development in Caenorhabditis elegans:: Uses and cautions

被引:18
作者
Maine, EM [1 ]
机构
[1] Syracuse Univ, Dept Biol, Syracuse, NY 13244 USA
关键词
Caenorhabditis elegans; C; elegans; RNA interference; RNAi; germ line; germ cells; development; germline development;
D O I
10.1006/dbio.2001.0394
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
RNA-mediated genetic interference (RNAi) has become a very useful tool for analyzing gene function in development and other processes. RNAi can be used as a complement to traditional genetic studies or as a primary means of determining biological function. However, the efficacy of RNAi depends on a variety of factors that the researcher must take into consideration. This review focuses on germline development in the nematode, Caenorhabditis elegans, and discusses the uses and limitations of RNAi in providing new information about gene function as well as the possible endogenous role RNAi plays in germline physiology. (C) 2001 Academic Press.
引用
收藏
页码:177 / 189
页数:13
相关论文
共 123 条
[1]   CONTROL OF THE SPERM-OOCYTE SWITCH IN CAENORHABDITIS-ELEGANS HERMAPHRODITES BY THE FEM-3 3' UNTRANSLATED REGION [J].
AHRINGER, J ;
KIMBLE, J .
NATURE, 1991, 349 (6307) :346-348
[2]  
Albertson DG, 1997, C ELEGANS, P47
[3]   RNA-mediated interference of a cdc25 homolog in Caenorhabditis elegans results in defects in the embryonic cortical membrane, meiosis, and mitosis [J].
Ashcroft, NR ;
Srayko, M ;
Kosinski, ME ;
Mains, PE ;
Golden, A .
DEVELOPMENTAL BIOLOGY, 1999, 206 (01) :15-32
[4]   GLP-1 IS REQUIRED IN THE GERM LINE FOR REGULATION OF THE DECISION BETWEEN MITOSIS AND MEIOSIS IN C-ELEGANS [J].
AUSTIN, J ;
KIMBLE, J .
CELL, 1987, 51 (04) :589-599
[5]   TRANSCRIPT ANALYSIS OF GLP-1 AND LIN-12, HOMOLOGOUS GENES REQUIRED FOR CELL-INTERACTIONS DURING DEVELOPMENT OF C-ELEGANS [J].
AUSTIN, J ;
KIMBLE, J .
CELL, 1989, 58 (03) :565-571
[6]  
BARTON MK, 1990, GENETICS, V125, P29
[7]   Role for a bidentate ribonuclease in the initiation step of RNA interference [J].
Bernstein, E ;
Caudy, AA ;
Hammond, SM ;
Hannon, GJ .
NATURE, 2001, 409 (6818) :363-366
[8]  
Bosher JM, 1999, GENETICS, V153, P1245
[9]  
Boxem M, 1999, DEVELOPMENT, V126, P2227
[10]   Cell division -: A histone-H3-like protein in C-elegans [J].
Buchwitz, BJ ;
Ahmad, K ;
Moore, LL ;
Roth, MB ;
Henikoff, S .
NATURE, 1999, 401 (6753) :547-548