Use of in vitro (Ames and Mutatox tests) and in vivo (Amphibian Micronucleus test) assays to assess the genotoxicity of leachates from a contaminated soil

被引:49
作者
Békaert, C
Rast, C
Ferrier, V
Bispo, A
Jourdain, MJ
Vasseur, P
机构
[1] Univ Toulouse 3, Ctr Dev Biol, INSERM, UMR 5547, F-31062 Toulouse, France
[2] Ctr Environm Sci, F-57040 Metz 1, France
[3] IRH Environm, F-54514 Vandoeuvre Nancy, France
关键词
genotoxicity; Ames test; Mutatox test; Amphibian Micronucleus test; polycyclic aromatic hydrocarbons; soil leachate;
D O I
10.1016/S0146-6380(99)00079-0
中图分类号
P3 [地球物理学]; P59 [地球化学];
学科分类号
0708 ; 070902 ;
摘要
Two in vitro bacterial assays (Ames test and Mutatox test) and one in vivo Micronucleus test on amphibians were used to assess the genotoxicity of aqueous leachates from a soil sample contaminated with polycyclic aromatic hydrocarbons (PAHs) and heavy metals. Positive results were obtained with the three tests: suggesting that this contaminated soil may be a threat to the aquatic environment. The test sensitivity was shown to be influenced by the experimental conditions. The response was higher with the nonfiltered leachate than with 0.45 mu m filtered one, which was the standard protocol (Norm X31-210). This standard procedure must be reconsidered in order to account for the pollutants adsorbed on solid particles which were biologically active. Incubation of bacteria in a liquid medium enhanced the sensitivity of the Ames test, by increasing the bioavailability of pollutants in comparison with the agar plate method. Biological tests appeared to be a useful complement to physico-chemical analysis, since they account for bioavailability and bioaccumulation of chemicals, and interactions between pollutants. (C) 1999 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:953 / 962
页数:10
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