A comparison of time-resolved fluoroimmunoassay and ELISA in the detection of casein in foodstuffs

被引：11

作者：

Sletten, GB ^{[1
]}

Lovberg, KE ^{[1
]}

Moen, LH ^{[1
]}

Skarpeid, HJ ^{[1
]}

Egaas, E ^{[1
]}

机构：

[1] Natl Vet Inst, N-0033 Oslo, Norway

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 2005年 / 16卷 / 3-4期

关键词：

casein; cow's milk allergy; food allergens; enzyme-linked immunosorbent assay; milk; time-resolved fluoroimmunoassay;

D O I：

10.1080/09540100500206020

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

With the aim of lowering the detection limit for casein in foods, three competitive assays are described: direct time-resolved fluoroimmunoassay (TR-FIA), using europium-conjugated antibody, indirect TR-FIA, using biotinylated antibody with europium-conjugated streptavidin and ELISA, using a HRP-conjugated secondary antibody. Food samples ( instant potato, flour mix, packet soup, spice-mix) were analysed. Standard curve sensitivities in direct and indirect TR-FIAs did not differ significantly ( p = 0.097), but both TR-FIAs were considerably less sensitive ( both p< 0.0001) than ELISA (LOQs 1.3, 1.5 and <1.0 mg kg(-1), respectively). The precision and working analyte range was similar in all three methods. Casein content measured in food products was comparable, using the three assays and rocket immunoelectrophoresis. The TR-FIA approach provided no improvement over the ELISA. All three assays allowed quantification of casein in foodstuffs in the order of 1 - 1.5 mg kg(-1), providing a basis for more rigorous validation and collaborative testing.

引用

页码：235 / 243

页数：9

共 24 条

[1] A competitive enzyme-linked immunosorbent assay for detection of bovine milk in ovine and caprine milk and cheese using a monoclonal antibody against bovine beta-casein [J].