Gene delivery in vitro and in vivo from bioreducible multilayered polyelectrolyte films of plasmid DNA

被引：113

作者：

Blacklock, Jenifer ^{[1
,2
]}

You, Ye-Zi ^{[1
]}

Zhou, Qing-Hui ^{[1
]}

Mao, Guangzhao ^{[3
]}

Oupicky, David ^{[1
]}

机构：

[1] Wayne State Univ, Dept Pharmaceut Sci, Detroit, MI 48202 USA

[2] Wayne State Univ, Dept Biomed Engn, Detroit, MI 48202 USA

[3] Wayne State Univ, Dept Chem Engn & Mat Sci, Detroit, MI 48202 USA

来源：

BIOMATERIALS | 2009年 / 30卷 / 05期

基金：

美国国家卫生研究院;

关键词：

Layer-by-layer; LBL; DNA; Polyelectrolyte films; In vivo transfection; PROTEIN DISULFIDE-ISOMERASE; CELL-SURFACE; THIN-FILMS; RELEASE; TRANSFECTION; COMPLEXES; BIOMATERIALS; SCAFFOLDS; LINKAGES; POLYMERS;

D O I：

10.1016/j.biomaterials.2008.10.012

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Layer-by-layer (LbL) films were assembled on flexible stainless steel substrate using plasmid DNA and reducible hyperbranched poly(amido amine) (RHB) polycation. The films were characterized by XPS and their disassembly in reducing conditions confirmed by ellipsometry. Fibroblast and smooth muscle cell attachment and proliferation on DNA/RHB films were indistinguishable from those on control DNA/poly(ethylenimine) (PEI) films. In vitro transfection activity was evaluated using reporter plasmids encoding for secreted alkaline phosphatase (SEAP) and green fluorescent protein (GFP). DNA/RHB films showed higher and longer lasting transfection activity than control DNA/PEI films using SEAP plasmid. It was revealed through the use of GFP plasmid that DNA/RHB films transfected almost the entire cell population growing on the films. In vivo transfection activity was evaluated by subcutaneously implanting a stainless steel substrate coated with the DNA/RHB films containing SEAP plasmid DNA and measuring the levels of SEAP secreted into the blood circulation of rats. It was found that the plasma levels of SEAP peaked at similar to 160 ng SEAP/mL five days post-implantation. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：939 / 950

页数：12

共 52 条

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