In vivo imaging reveals an essential role for neutrophils in leishmaniasis transmitted by sand flies

被引：628

作者：

Peters, Nathan C. ^{[2
]}

Egen, Jackson G. ^{[1
]}

Secundino, Nagila ^{[2
]}

Debrabant, Alain ^{[3
]}

Kimblin, Nicola ^{[2
]}

Kamhawi, Shaden ^{[2
]}

Lawyer, Phillip ^{[2
]}

Fay, Michael P. ^{[4
]}

Germain, Ronald N. ^{[1
]}

Sacks, David ^{[2
]}

机构：

[1] NIAID, Immunol Lab, Bethesda, MD 20892 USA

[2] NIAID, Parasit Dis Lab, Bethesda, MD 20892 USA

[3] US FDA, Lab Bacterial Parasit & Unconvent Agents, Div Emerging & Transfus Transmitted Dis, Ctr Biol Evaluat & Res, Bethesda, MD 20892 USA

[4] NIAID, Biostat Res Branch, Bethesda, MD 20892 USA

来源：

SCIENCE | 2008年 / 321卷 / 5891期

关键词：

D O I：

10.1126/science.1159194

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Infection with the obligate intracellular protozoan Leishmania is thought to be initiated by direct parasitization of macrophages, but the early events following transmission to the skin by vector sand flies have been difficult to examine directly. Using dynamic intravital microscopy and flow cytometry, we observed a rapid and sustained neutrophilic infiltrate at localized sand fly bite sites. Invading neutrophils efficiently captured Leishmania major ( L. m.) parasites early after sand fly transmission or needle inoculation, but phagocytosed L. m. remained viable and infected neutrophils efficiently initiated infection. Furthermore, neutrophil depletion reduced, rather than enhanced, the ability of parasites to establish productive infections. Thus, L. m. appears to have evolved to both evade and exploit the innate host response to sand fly bite in order to establish and promote disease.

引用

页码：970 / 974

页数：5

共 26 条

[1] Inhibition of the spontaneous apoptosis of neutrophil granulocytes by the intracellular parasite Leishmania major [J].