Role of transmembrane semaphorin Sema6A in oligodendrocyte differentiation and myelination

被引：45

作者：

Bernard, Frederic ^{[2
]}

Moreau-Fauvarque, Caroline ^{[1
,2
,3
,4
]}

Heitz-Marchaland, Celine ^{[1
,2
,3
,4
]}

Zagar, Yvrick ^{[1
,2
,3
,4
]}

Dumas, Laura ^{[1
,2
,3
,4
]}

Fouquet, Stephane ^{[1
,3
,4
]}

Lee, Xinhua ^{[5
]}

Shao, Zhaohui ^{[5
]}

Mi, Sha ^{[5
]}

Chedotal, Alain ^{[1
,2
,3
,4
,5
,6
]}

机构：

[1] INSERM, Inst Vis, UMRS 968, Paris, France

[2] CNRS, UMR 7102, Paris, France

[3] INSERM, U968, Paris, France

[4] Univ Paris 06, UPMC, UMR S 968, Inst Vis, Paris, France

[5] Biogen Idec Inc, Dept Discovery Biol, Cambridge, MA USA

[6] Grp Hosp Pitie Salpetriere, AP HP, F-75634 Paris, France

来源：

GLIA | 2012年 / 60卷 / 10期

关键词：

semaphorin; plexin; myelination; oligodendrocyte; PROTEOLIPID PROTEIN; DISTINCT FUNCTIONS; MURINE SEMAPHORIN; STRUCTURAL BASIS; NOGO-A; EXPRESSION; PLEXIN; MOUSE; IDENTIFICATION; REMYELINATION;

D O I：

10.1002/glia.22378

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Myelination is regulated by extracellular proteins, which control interactions between oligodendrocytes and axons. Semaphorins are repulsive axon guidance molecules, which control the migration of oligodendrocyte precursors during normal development and possibly in demyelinating diseases. We show here that the transmembrane semaphorin 6A (Sema6A) is highly expressed by myelinating oligodendrocytes in the postnatal mouse brain. In adult mice, Sema6A expression is upregulated in demyelinating lesions in cuprizone-treated mice. The analysis of the optic nerve and anterior commissure of Sema6A-deficient mice revealed a marked delay of oligodendrocyte differentiation. Accordingly, the development of the nodes of Ranvier is also transiently delayed. We also observed an arrest in the in vitro differentiation of purified oligodendrocytes lacking Sema6A, with a reduction of the expression level of Myelin Basic Protein. Their morphology is also abnormal, with less complex and ramified processes than wild-type oligodendrocytes. In myelinating co-cultures of dorsal root ganglion neurons and purified oligodendrocytes we found that myelination is perturbed in absence of Sema6A. These results suggest that Sema6A might have a role in myelination by controlling oligodendrocyte differentiation. (c) 2012 Wiley Periodicals, Inc

引用

页码：1590 / 1604

页数：15

共 71 条

[1] Expression of Semaphorin 4F in neurons and brain oligodendrocytes and the regulation of oligodendrocyte precursor migration in the optic nerve [J].