Determination of flunixin in equine urine and serum by capillary electrophoresis

被引：19

作者：

Gu, XL

MelekaBoules, M

Chen, CL

Ceska, DM

Tiffany, DM

机构：

[1] UNIV FLORIDA,COLL VET MED,DEPT LARGE ANIM CLIN SCI,GAINESVILLE,FL 32610

[2] DEPT BUSINESS & PROFESS REGULAT FLORIDA,DIV PARIMUTUEL WAGERING,TALLAHASSEE,FL 32399

来源：

JOURNAL OF CHROMATOGRAPHY B | 1997年 / 692卷 / 01期

关键词：

flunixin;

D O I：

10.1016/S0378-4347(96)00393-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A capillary electrophoresis (CE) and a solid-phase extraction method was developed for the determination of flunixin in equine urine and serum. The suitable CE run conditions were described. The factors affecting flunixin recovery rates were investigated and optimum solid-phase extraction conditions for flunixin in equine urine and serum were established. Limits of detection and quantitation were 3.4 and 5.6 ng/ml for serum and 16.9 and 33.1 ng/ml for urine, respectively. The recoveries exceeded 96% far urine and 79% for serum. Urine samples from race horses and urine and serum samples from a mare administrated with flunixin were analyzed with this procedure.

引用

页码：187 / 198

页数：12

共 16 条

[1] ANDERSON KL, 1986, AM J VET RES, V47, P1366
[2] BOTTOMS GD, 1981, AM J VET RES, V42, P1514
[3] CHAY S, 1982, Equine Practice, V4, P16
[4] CIOFALO VB, 1977, J PHARMACOL EXP THER, V200, P501
[5] EVALUATION OF THE POTENTIAL FOR INTERFERENCE BY DIMETHYLSULFOXIDE (DMSO) IN DRUG DETECTION IN RACING ANIMALS
CRAIG, AM
BLYTHE, LL
APPELL, LH
SLIZESKI, ML
[J]. JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS, 1987, 10 (04) : 298 - 304
[6] FESSLER JF, 1982, AM J VET RES, V43, P140
[7] SIMULTANEOUS DETERMINATION OF FLUNIXIN, PHENYLBUTAZONE, OXYPHENBUTAZONE AND GAMMA-HYDROXYPHENYLBUTAZONE IN EQUINE PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - WITH APPLICATION TO PHARMACOKINETICS
HARDEE, GE
LAI, JW
MOORE, JN
[J]. JOURNAL OF LIQUID CHROMATOGRAPHY, 1982, 5 (10): : 1991 - 2003
[8] MEASUREMENT OF FLUNIXIN IN EQUINE INFLAMMATORY EXUDATE AND PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
HIGGINS, AJ
LEES, P
SHARMA, SC
TAYLOR, JB
[J]. EQUINE VETERINARY JOURNAL, 1987, 19 (04) : 303 - 306
[9] HOUDESHELL JW, 1977, J EQUINE MED SURG, V1, P57
[10] IDENTIFICATION OF A FLUNIXIN METABOLITE IN THE HORSE BY GAS CHROMATOGRAPHY-MASS SPECTROMETRY
JAUSSAUD, P
COURTOT, D
GUYOT, JL
PARIS, J
[J]. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1987, 423 : 123 - 130

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