Effect of the crystallizing agent 2-methyl-2,4-pentanediol on the structure of adenine tract DNA in solution

被引：25

作者：

Ganunis, RM ^{[1
]}

Guo, H ^{[1
]}

Tullius, TD ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,DEPT CHEM,BALTIMORE,MD 21218

来源：

BIOCHEMISTRY | 1996年 / 35卷 / 43期

关键词：

D O I：

10.1021/bi961880q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The hydroxyl radical cleavage pattern of bent DNA containing phased adenine tracts has a sinusoidal pattern. Changes in the cleavage pattern of the sequence A(5)N(5) in the presence of 2-methyl-2,4-pentanediol (MPD) demonstrate that MPD has an effect on the structure of a bent A-tract region of DNA. The effect of MPD on DNA structure appears to be limited to bent A-tract regions, as we find no significant changes in the cleavage pattern of flanking regions, mixed-sequence DNA, or the unbent A-tract sequence T(4)A(4)N(2).

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收藏

页码：13729 / 13732

页数：4

相关论文

共 15 条

[1] THE UNUSUAL CONFORMATION ADOPTED BY THE ADENINE TRACTS IN KINETOPLAST DNA [J].

BURKHOFF, AM ;

TULLIUS, TD .

CELL, 1987, 48 (06) :935-943

[2] STRUCTURAL DETAILS OF AN ADENINE TRACT THAT DOES NOT CAUSE DNA TO BEND [J].

BURKHOFF, AM ;

TULLIUS, TD .

NATURE, 1988, 331 (6155) :455-457

[3] MPD and DNA bending in crystals and in solution [J].

Dickerson, RE ;

Goodsell, D ;

Kopka, ML .

JOURNAL OF MOLECULAR BIOLOGY, 1996, 256 (01) :108-125

[4] A DNA DODECAMER CONTAINING AN ADENINE TRACT CRYSTALLIZES IN A UNIQUE LATTICE AND EXHIBITS A NEW BEND [J].

DIGABRIELE, AD ;

STEITZ, TA .

JOURNAL OF MOLECULAR BIOLOGY, 1993, 231 (04) :1024-1039

[5] The organic crystallizing agent 2-methyl-2,4-pentanediol reduces DNA curvature by means of structural changes in A-tracts [J].

Dlakic, M ;

Park, K ;

Griffith, JD ;

Harvey, SC ;

Harrington, RE .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (30) :17911-17919

[6] SEQUENCE-DIRECTED CURVATURE OF DNA [J].

HAGERMAN, PJ .

NATURE, 1986, 321 (6068) :449-450

[7] SEQUENCE DEPENDENCE OF THE CURVATURE OF DNA - A TEST OF THE PHASING HYPOTHESIS [J].

HAGERMAN, PJ .

BIOCHEMISTRY, 1985, 24 (25) :7033-7037

[8] WHAT IS THE BASIS OF SEQUENCE-DIRECTED CURVATURE IN DNAS CONTAINING A-TRACTS [J].

HARVEY, SC ;

DLAKIC, M ;

GRIFFITH, J ;

HARRINGTON, R ;

PARK, K ;

SPROUS, D ;

ZACHARIAS, W .

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 1995, 13 (02) :301-307

[9] TWO-DIMENSIONAL NMR INVESTIGATION OF A BENT DNA FRAGMENT - ASSIGNMENT OF THE PROTON RESONANCES AND PRELIMINARY STRUCTURE-ANALYSIS [J].

KINTANAR, A ;

KLEVIT, RE ;

REID, BR .

NUCLEIC ACIDS RESEARCH, 1987, 15 (14) :5845-5862

[10] DNA BENDING AT ADENINE . THYMINE TRACTS [J].

KOO, HS ;

WU, HM ;

CROTHERS, DM .

NATURE, 1986, 320 (6062) :501-506