Receptor-mediated activation of phospholipase D by sphingosine 1-phosphate in skeletal muscle C2C12 cells - A role for protein kinase C

被引：46

作者：

Meacci, E ^{[1
]}

Vasta, V ^{[1
]}

Donati, C ^{[1
]}

Farnararo, M ^{[1
]}

Bruni, P ^{[1
]}

机构：

[1] Univ Florence, Dipartimento Sci Biochim, I-50134 Florence, Italy

来源：

FEBS LETTERS | 1999年 / 457卷 / 02期

关键词：

phospholipase D; sphingosine; 1-phosphate; protein kinase C; C2C12; myoblast; Edg receptor;

D O I：

10.1016/S0014-5793(99)01033-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study showed that sphingosine 1-phosphate (SPP) induced rapid stimulation of phospholipase D (PLD) in skeletal muscle C2C12 cells. The effect was receptor-mediated since it was fully inhibited by pertussis toxin. AU known SPP-specific receptors, Edg-1, Edg-3 and AGR16/H218, resulted to be expressed in C2C12 myoblasts, although at a different extent. SPP-induced PLD activation did not involve membrane translocation of PLD1 or PLD2 and appeared to be fully dependent on protein kinase C (PKC) catalytic activity, SPP increased membrane association of PKC alpha, PKC delta and PKC lambda, however, only PKC alpha and PKC delta played a role in PLD activation since low concentrations of GF109203X and rottlerin, a selective inhibitor of PKC delta, prevented PLD stimulation, (C) 1999 Federation of European Biochemical Societies.

引用

页码：184 / 188

页数：5

共 26 条

[1] Translocation of protein kinase Cε and protein kinase Cδ to membrane is required for ultraviolet B-induced activation of mitogen-activated protein kinases and apoptosis [J].

Chen, NY ;

Ma, WY ;

Huang, CS ;

Dong, ZG .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (22) :15389-15394

[2] Phospholipase D2, a distinct phospholipase D isoform with novel regulatory properties that provokes cytoskeletal reorganization [J].

Colley, WC ;

Sung, TC ;

Roll, R ;

Jenco, J ;

Hammond, SM ;

Altshuller, Y ;

BarSagi, D ;

Morris, AJ ;

Frohman, MA .

CURRENT BIOLOGY, 1997, 7 (03) :191-201

[3] Phospholipase D: Enzymology, mechanisms of regulation, and function [J].

Exton, JH .

PHYSIOLOGICAL REVIEWS, 1997, 77 (02) :303-320

[4]

Facchinetti MM, 1998, J LIPID RES, V39, P197

[5] Diversity of cellular receptors and functions for the lysophospholipid growth factors lysophosphatidic acid and sphingosine 1-phosphate [J].

Goetzl, EJ ;

An, SZ .

FASEB JOURNAL, 1998, 12 (15) :1589-1598

[6] The novel sphingosine 1-phosphate receptor AGR16 is coupled via pertussis toxin-sensitive and -insensitive G-proteins to multiple signalling pathways [J].

Gonda, K ;

Okamoto, H ;

Takuwa, N ;

Yatomi, Y ;

Okazaki, H ;

Sakurai, T ;

Kimura, S ;

Sillard, R ;

Harii, K ;

Takuwa, Y .

BIOCHEMICAL JOURNAL, 1999, 337 :67-75

[7] ROTTLERIN, A NOVEL PROTEIN-KINASE INHIBITOR [J].

GSCHWENDT, M ;

MULLER, HJ ;

KIELBASSA, K ;

ZANG, R ;

KITTSTEIN, W ;

RINCKE, G ;

MARKS, F .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 199 (01) :93-98

[8] Characterization of the regulation of phospholipase D activity in the detergent-insoluble fraction of HL60 cells by protein kinase C and small G-proteins [J].

Hodgkin, MN ;

Clark, JM ;

Rose, S ;

Saqib, K ;

Wakelam, MJO .

BIOCHEMICAL JOURNAL, 1999, 339 :87-93

[9] Association of phospholipase D activity with the detergent-insoluble cytoskeleton of U937 promonocytic leukocytes [J].

Iyer, SS ;

Kusner, DJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (04) :2350-2359

[10] Extracellular sphingosine 1-phosphate stimulates formation of ethanolamine from phosphatidylethanolamine: Modulation of sphingosine 1-phosphate-induced mitogenesis by ethanolamine [J].

Kiss, Z ;

Crilly, KS ;

Anderson, WH .

BIOCHEMICAL JOURNAL, 1997, 328 :383-391

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