Detection and isolation of nuclear haplotypes by PCR-SSCP

被引：47

作者：

Orti, G

Hare, MP

Avise, JC

机构：

[1] Department of Genetics, University of Georgia, Athens

[2] Dept. of Organismic and Evol. Biol., Harvard University Biolabs, Cambridge, MA 02138

[3] Laboratory of John Avise, University of Georgia

[4] S. Palumbi, Harvard University

来源：

MOLECULAR ECOLOGY | 1997年 / 6卷 / 06期

关键词：

SSCP; allelic variation; nuclear haplotypes; gene genealogies; Limulus; Crassostrea;

D O I：

10.1046/j.1365-294X.1997.00212.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SSCP (single-strand conformational polymorphism) is used widely in the field of human biomedicine, but its potential as a population genetics tool for the recovery of nuclear gene genealogies remains to be realized. We describe and illustrate a use for SSCP in the physical isolation of nuclear haplotypes that circumvents several difficulties associated with more conventional cloning procedures. The DNA sequence can be determined directly from the isolated haplotypes and used for phylogenetic inference. SSCP provides a convenient first step toward generating nuclear genealogies for population studies.

引用

页码：575 / 580

页数：6

共 33 条

[1]

AQUADRO CF, 1986, GENETICS, V114, P1165

[2] GENE TREES AND ORGANISMAL HISTORIES - A PHYLOGENETIC APPROACH TO POPULATION BIOLOGY [J].

AVISE, JC .

EVOLUTION, 1989, 43 (06) :1192-1208

[3]

AVISE JC, 1987, ANNU REV ECOL SYST, V18, P489, DOI 10.1146/annurev.es.18.110187.002421

[4]

Avise John C., 1994, pi

[5] CONCORDANT MITOCHONDRIAL AND NUCLEAR-DNA PHYLOGENIES FOR POPULATIONS OF THE TELEOST FISH FUNDULUS-HETEROCLITUS [J].

BERNARDI, G ;

SORDINO, P ;

POWERS, DA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (20) :9271-9274

[6] MOST CLASSICAL MUS-MUSCULUS-DOMESTICUS LABORATORY MOUSE STRAINS CARRY A MUS-MUSCULUS-MUSCULUS Y-CHROMOSOME [J].

BISHOP, CE ;

BOURSOT, P ;

BARON, B ;

BONHOMME, F ;

HATAT, D .

NATURE, 1985, 315 (6014) :70-72

[7] OPTIMIZATION OF THE SINGLE-STRAND CONFORMATION POLYMORPHISM (SSCP) TECHNIQUE FOR DETECTION OF POINT MUTATIONS [J].

GLAVAC, D ;

DEAN, M .

HUMAN MUTATION, 1993, 2 (05) :404-414

[8] CLONAL DIVERGENCE IN ESCHERICHIA-COLI AS A RESULT OF RECOMBINATION, NOT MUTATION [J].

GUTTMAN, DS ;

DYKHUIZEN, DE .

SCIENCE, 1994, 266 (5189) :1380-1383

[9] GENERATION OF SINGLE-STRANDED-DNA BY THE POLYMERASE CHAIN-REACTION AND ITS APPLICATION TO DIRECT SEQUENCING OF THE HLA-DQA LOCUS [J].

GYLLENSTEN, UB ;

ERLICH, HA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (20) :7652-7656

[10] Anonymous nuclear DNA markers in the American oyster and their implications for the heterozygote deficiency phenomenon in marine bivalves [J].

Hare, MP ;

Karl, SA ;

Avise, JC .

MOLECULAR BIOLOGY AND EVOLUTION, 1996, 13 (02) :334-345

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