Induction of polymethylgalacturonase biosynthesis by immobilized cells of Aspergillus niger 26

被引：12

作者：

Pashova, S ^{[1
]}

Slokoska, L ^{[1
]}

Krumova, E ^{[1
]}

Angelova, M ^{[1
]}

机构：

[1] Bulgarian Acad Sci, Inst Microbiol, BU-1113 Sofia, Bulgaria

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1999年 / 24卷 / 8-9期

关键词：

Aspergillus niger; immobilized cells; polymethylgalacturonase; pectinase; regulation; induction;

D O I：

10.1016/S0141-0229(98)00152-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The involvement of the regulatory mechanism induction in the biosynthesis of polymethylgalacturonase (PMG) by immobilized cells of Aspergillus niger 26 was studied. The immobilization altered the spectrum of pectin-degrading enzymes. The free cell cultures produced four pectinolytic enzyme activities, namely PMC, polygalacturonase (PG), pectinesterase (PE) and pectinylase (PL),while the entrapped mycelium synthesized PMG and PG only. The best inducer for PMG synthesis was highly esterified apple pectin. The effects of various pectin concentrations and time of incubation with inducer showed the induction model of PMG biosynthesis. The deinduction experiments provided evidence that the presence of the inducer in every cycle was obligatory for the PMG biosynthesis during repeated replacement cultivation. (C) 1999 Elsevier Science me. All rights reserved.

引用

页码：535 / 540

页数：6

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